Pfu DNA Polymerase

EMQZ1401

Pfu DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from E.coli with proofreading activity (Pyrococcus furiosis DNA Polymerase gene). Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Pfu DNA Polymerase provides much higher fidelity than Taq DNA polymerase when doing PCR of problematic and / or GC-rich templates. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

• High-fidelity PCR and primer-extension reactions• Generation of PCR products for cloning and expression• PCR cloning and blunt-end amplification product Generation• RT-PCR for cDNA cloning and expression• Site-directed mutagenesis• blunt-end PCR cloning

2,500 U/ml

10×Pfu DNA Polymerase Reaction Buffer (containing 20 mM MgSO4): [200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM (NH4)2SO4, 100 mM KCl, 1 mg/ml BSA (without nulease activity), 1% Triton X-100 (V/V), 20 mM MgSO4].

100 U; 500 U

An E.coli strain that carries the pfu DNA polymerase gene from Pyrococcus furiosus.

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 72°C.