DNA Polymerase I (E.coli)

Name: DNA Polymerase I (E.coli)
SKU: EMQZ1403
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1403

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Product Information

Cat. No.	EMQZ1403
Description	DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity. The combination of DNA synthesis and 5' -> 3' nuclease characteristics enable nick-translation during DNA synthesis.
Source	A recombinant E. coli strain carrying the PolA gene.
Concentration	10,000 U/ml
Applications	• Nick translation of DNA to obtain probes with a High specific activity • Second strand synthesis of cDNA
Size	500 U; 2,500 U
Unit Definition	One unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
Reaction Conditions	1×DNA Polymerase I Reaction System containing: 1×DNA Polymerase I Reaction Buffer［10 mM Tris-HCl (pH 7.9 at 25°C), 50 mM NaCl, 10 mM MgCl2, 1 mM DTT］; add dNTPs (not supplied with enzyme); incubate at 37°C.
Storage	Store at -20°C

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Q & A

Customer Reviews

Customer Q&As

Does the enzyme have 3'→5' exonuclease activity?

A: Yes, the enzyme has single-stranded specific 3'→5' exonuclease (proofreading) activity and double-stranded specific 5'→3' exonuclease activity.

How is the enzyme activity of this enzyme defined?

A: One unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.

Can only DNA primers be used when amplifying with this enzyme?

A: No, the template for amplification must be DNA, but the primers can be either DNA or RNA.

Can site-directed mutagenesis be achieved when using the enzyme alone?

A: No, it cannot. Since the enzyme lacks endonuclease activity, it cannot exhibit site-specific nicking activity when used alone.

Is it better to add more enzyme during the reaction?

A: No, excessive use of the enzyme can lead to aggregation due to its strong affinity for DNA, which can prevent the reaction from proceeding fully. Therefore, it is recommended to add the appropriate amount of enzyme according to the instructions.

Can it be melted at room temperature for use?

A: Not recommended. Although the enzyme activity is stable, it is generally recommended to thaw on ice before use. Thawing at room temperature may cause other issues.

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Customer Reviews

Horizontal incision

It can be used together with DNase I for site-directed mutagenesis.

United States

2023-03-31

DNA probe

The enzyme was successfully used to prepare probes with high specific activity.

United States

2021-01-02

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DNA Polymerase I (E.coli)

Product Information

Q & A

Customer Reviews

Does the enzyme have 3'→5' exonuclease activity?

How is the enzyme activity of this enzyme defined?

Can only DNA primers be used when amplifying with this enzyme?

Can site-directed mutagenesis be achieved when using the enzyme alone?

Is it better to add more enzyme during the reaction?

Can it be melted at room temperature for use?

Horizontal incision

DNA probe

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