DNA Polymerase I (E.coli)

EMQZ1403

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity. The combination of DNA synthesis and 5' -> 3' nuclease characteristics enable nick-translation during DNA synthesis.

• Nick translation of DNA to obtain probes with a High specific activity• Second strand synthesis of cDNA

10,000 U/ml

1×DNA Polymerase I Reaction System containing: 1×DNA Polymerase I Reaction Buffer［10 mM Tris-HCl (pH 7.9 at 25°C), 50 mM NaCl, 10 mM MgCl2, 1 mM DTT］; add dNTPs (not supplied with enzyme); incubate at 37°C.

500 U; 2,500 U

A recombinant E. coli strain carrying the PolA gene.

Store at -20°C

One unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.