Tli (exo-) DNA polymerase

EMQZ1409

Tli (exo-) DNA Polymerase, also called Vent (exo-) DNA Polymerase, has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity. This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase.

• Applications• PCR• Primer extension• Thermal cycle sequencing• High temperature dideoxy-sequencing

2,000 U/ml

100 ul Reaction System containing: 1×Tli (exo-) DNA Polymerase Buffer[20 mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; primers; dNTPs; 2-4 U polymerase.

100 U; 500 U

Purified from a recombinant E. coli that carries the (D141A / E143A) DNA Polymerase gene

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.