Tli (exo-) DNA polymerase

Name: Tli (exo-) DNA polymerase
SKU: EMQZ1409
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1409

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Product Information

Cat. No.	EMQZ1409
Description	Tli (exo-) DNA Polymerase, also called Vent (exo-) DNA Polymerase, has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity. This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase.
Source	Purified from a recombinant E. coli that carries the (D141A / E143A) DNA Polymerase gene
Concentration	2,000 U/ml
Applications	• Applications • PCR • Primer extension • Thermal cycle sequencing • High temperature dideoxy-sequencing
Size	100 U; 500 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Reaction Conditions	100 ul Reaction System containing: 1×Tli (exo-) DNA Polymerase Buffer[20 mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; primers; dNTPs; 2-4 U polymerase.
Storage	Store at -20°C

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Customer Reviews

Customer Q&As

How is the fidelity of Tli (exo-) DNA Polymerase?

A: Tli (exo-) DNA Polymerase lacks 3'→5' exonuclease activity, which results in a slight decrease in fidelity.

What is the source of Tli (exo-) DNA Polymerase?

A: It is derived from Thermococcus litoralis and has undergone genetic engineering modifications.

Can the buffer provided with this product be used if it has precipitated?

A: The 10× HG PCR Buffer 1 has a higher concentration and may exhibit white precipitation, but after thorough mixing, it does not affect its usability.

How efficient is the amplification with Tli (exo-) DNA Polymerase?

A: It is suitable for amplifying 5kb DNA fragments using λDNA as a template and 2kb DNA fragments using the human genome DNA as a template. The amplification rate is 2kb/min.

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