Hot Start Taq DNA Polymerase

Name: Hot Start Taq DNA Polymerase
SKU: EMDT0316
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMDT0316

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Product Information

Cat. No.	EMDT0316
Description	Phoenix Hot Start Taq DNA Polymerase is a recombinant, thermostabile Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the 5′→3′ polymerase activity prior to the initial DNA denaturation step of PCR. Such antibody-mediated Hot-Start capability enhances the overall specificity, sensitivity and yield of the PCR by reducing nonspecific amplification and primer-dimer formation prior to PCR cycling, and allows the convenience of reaction set up at room temperature. When the temperature of the PCR reaction mix reaches ≥94 °C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. Phoenix Hot Start Taq DNA Polymerase, like standard Taq DNA polymerase, also has 5′→3′ exonuclease activity, but lacks any detectable 3′→5′ exonuclease activity.
Source	A recombinant E. coli strain carrying the Taq DNA polymerase gene from the thermophilic organism Thermus Aquaticus YT-1 complexed with a monoclonal antibody derived from murine cell culture.
Concentration	5000 U/ml
Size	500 Units
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75 °C.
Storage	20 mM Tris-HCl100 mM NaCl0.1 mM EDTAStabilizer50% GlycerolpH 7.5 at 25 °C

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Customer Q&As

What are the activities of this enzyme as an exo-enzyme?

A: This enzyme has 5′→3′ exonuclease activity but lacks 3′→5′ exonuclease activity.

What is the amplification specificity of the Hot Start Taq DNA Polymerase?

A: The enzyme complex contains corresponding antibodies, which mediate the hot-start capability by reducing nonspecific amplification and primer dimer formation before PCR cycling, thereby improving the overall specificity, sensitivity, and yield of PCR.

Does the Hot Start Taq DNA Polymerase exhibit activity at room temperature?

A: This enzyme is not active at room temperature to avoid primer dimer formation and nonspecific extension. Its activity can be restored by heating at 95°C for 4 minutes.

When using this enzyme for amplification, does it add extra bases to the ends of the target product?

A: Yes, the 3'-end of the PCR product will have an additional adenosine (dA), resulting in a 3'-dA overhang in the target fragment.

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Customer Reviews

High specificity

When using this enzyme for amplifying target fragments, there are very few primer dimers formed, resulting in high product specificity.

United States

2023-04-11

High stability

The reaction system can be prepared at room temperature without affecting the enzyme activity.

United States

2020-08-23

Well-packaged product

The Hot Start Taq DNA Polymerase is well-packaged and transported, and there are no quality issues with the product.

United States

2021-05-12

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Hot Start Taq DNA Polymerase

Product Information

Q & A

Customer Reviews

What are the activities of this enzyme as an exo-enzyme?

What is the amplification specificity of the Hot Start Taq DNA Polymerase?

Does the Hot Start Taq DNA Polymerase exhibit activity at room temperature?

When using this enzyme for amplification, does it add extra bases to the ends of the target product?

High specificity

High stability

Well-packaged product

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