DNA Polymerase I Large Fragment (Klenow)

Name: DNA Polymerase I Large Fragment (Klenow)
SKU: EMQZ1402
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1402

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Product Information

Cat. No.	EMQZ1402
Description	DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Source	Purified from a strain of E. coli that carries the DNA Polymerase I, Large (Klenow) Fragment gene.
Concentration	5,000 U/ml
Applications	• DNA sequencing by the Sanger dideoxy method • Fill-in of 5´ overhangs to form blunt ends • Removal of 3´ overhangs to form blunt ends • Second strand cDNA synthesis • Second strand synthesis in mutagenesis protocols
Size	200 U; 1,000 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 72°C.
Reaction Conditions	1×Klenow Reaction System containing: 1×Klenow Reaction Buffer [25 mM Tric-HCl (pH 7.4 at 25°C), 0.1 mM EDTA,1 mM Dithiothreitol (DTT), 50% glycerol (V/V)]; add dNTPs (not supplied with enzyme); incubate at 37°C.
Storage	Store at -20°C

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Customer Q&As

Does DNA Polymerase I Large Fragment (Klenow) have proofreading activity?

A: DNA Polymerase I Large Fragment (Klenow) is a DNA polymerase with high fidelity but lacks proofreading activity.

Can DNA Polymerase I Large Fragment (Klenow) blunt-end ligate 5' or 3' overhang molecules?

A: Yes, it can.

Is the fidelity of DNA Polymerase I Large Fragment (Klenow) affected by base analogs?

A: To achieve the highest fidelity, we recommend adding low concentrations of base analogs in the reaction.

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