DNA Polymerase I Large Fragment (Klenow)

EMQZ1402

DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.

• DNA sequencing by the Sanger dideoxy method• Fill-in of 5´ overhangs to form blunt ends• Removal of 3´ overhangs to form blunt ends• Second strand cDNA synthesis• Second strand synthesis in mutagenesis protocols

5,000 U/ml

1×Klenow Reaction System containing: 1×Klenow Reaction Buffer [25 mM Tric-HCl (pH 7.4 at 25°C), 0.1 mM EDTA,1 mM Dithiothreitol (DTT), 50% glycerol (V/V)]; add dNTPs (not supplied with enzyme); incubate at 37°C.

200 U; 1,000 U

Purified from a strain of E. coli that carries the DNA Polymerase I, Large (Klenow) Fragment gene.

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 72°C.