Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMQZ1408
| Cat. No. | EMQZ1408 |
| Description | Tli DNA Polymerase (also called Vent DNA Polymerase) is a high-fidelity thermophilic DNA polymerase. The fidelity of Tli DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase. This high fidelity derives in part from an integral 3''→5'' proofreading exonuclease activity in Thi DNA Polymerase. Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95°C. |
| Source | Purified from a recombinant E. coli strain carries Thi DNA Polymerase gene. |
| Concentration | 2,000 U/ml |
| Applications |
• PCR • Primer extension |
| Size | 100 U; 500 U |
| Unit Definition | One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. |
| Reaction Conditions | 100 ul Reaction System containing: 1×Tli DNA Polymerase Reaction Buffer ( 20mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; dNTPs; primers; 1-2 U polymerase. |
| Storage | Store at -20°C |
A: Not recommended, excessive cycles can lead to product smearing.
A: The Tli DNA polymerase is derived from Thermococcus litoralis and obtained through genetic engineering.
A: No, Tli DNA polymerase is intended for basic research purposes only and should not be used for human or animal therapy, clinical diagnosis, or as an additive in food, cosmetics, or household products.
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Tli DNA polymerase still has over 90% polymerase activity after being incubated at 95°C for 1 hour.
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