Psp GBD DNA polymerase

Name: Psp GBD DNA polymerase
SKU: EMQZ1410
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1410

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Product Information

Cat. No.	EMQZ1410
Description	Psp GBD DNA Polymerase, also called Deep Vent DNA Polymerase, is a high-fidelity thermophilic DNA polymerase. This high fidelity derives in part from an integral 3'→5' proofreading exonuclease activity. Psp GBD DNA Polymerase is more stable than Tli DNA Polymerase at 95°C to 100°C. The half-life of Psp GBD DNA Polymerase is 8 hours at 100°C.
Source	Purified from a recombinant E. coli that carries Psp GBD DNA Polymerase gene from Pyrococcus species GB-D.
Concentration	2,000 U/ml
Applications	• PCR • Primer extension
Size	100 U; 500 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Reaction Conditions	100 ul Reaction System containing: 1×Psp GBD DNA Polymerase Buffer[20 mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; primers; dNTPs; 1-2 U polymerase.
Storage	Store at -20°C

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Q & A

Customer Reviews

Customer Q&As

How is the enzyme activity defined?

A: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

How to prevent liquid evaporation?

A: The heated lid function of the PCR machine can prevent liquid evaporation. If the PCR machine does not have this function, a small amount of paraffin oil can be added to the liquid surface to prevent evaporation.

Can 40 cycles be performed during PCR amplification?

A: It is not recommended to cycle the product more than 20-30 times as the fidelity of the product decreases with increased cycling.

What is the appropriate amount of the enzyme to add when amplifying the target gene in PCR?

A: We recommend adding 2-4 uL Psp GBD DNA polymerase per 100 uL reaction system.

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Customer Reviews

Good stability

Psp GBD DNA polymerase is more stable than Tli DNA polymerase at 95°C to 100°C.

United States

2021-11-21

Good service

Both pre-sales and after-sales services are excellent, and the staff are patient in answering questions.

United States

2022-01-31

Wide range of applications

It can not only be used for primer amplification but also for high-temperature (72°C) sequencing.

United States

2022-08-05

Long half-life

At 95℃, the half-life of Psp GBD DNA polymerase is 23 hours, Tli DNA polymerase is 6.7 hours, and Taq DNA polymerase is 1.6 hours.

United States

2023-03-15

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Psp GBD DNA polymerase

Product Information

Q & A

Customer Reviews

How is the enzyme activity defined?

How to prevent liquid evaporation?

Can 40 cycles be performed during PCR amplification?

What is the appropriate amount of the enzyme to add when amplifying the target gene in PCR?

Good stability

Good service

Wide range of applications

Long half-life

Related Products

Related Services