Psp GBD DNA polymerase

EMQZ1410

Psp GBD DNA Polymerase, also called Deep Vent DNA Polymerase, is a high-fidelity thermophilic DNA polymerase. This high fidelity derives in part from an integral 3'→5' proofreading exonuclease activity. Psp GBD DNA Polymerase is more stable than Tli DNA Polymerase at 95°C to 100°C. The half-life of Psp GBD DNA Polymerase is 8 hours at 100°C.

• PCR• Primer extension

2,000 U/ml

100 ul Reaction System containing: 1×Psp GBD DNA Polymerase Buffer[20 mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; primers; dNTPs; 1-2 U polymerase.

100 U; 500 U

Purified from a recombinant E. coli that carries Psp GBD DNA Polymerase gene from Pyrococcus species GB-D.

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.