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Human DNA Polymerase Beta

Exclusively from CHIMERx, Human DNA Polymerase Beta is a repair enzyme used to fill gaps and nicks in double-stranded DNA
Repair polymerase able to synthesize DNA beyond the end of a gap or nick with simultaneous displacement of the non-replicated strand.Fills gaps and nicks.
Reaction Buffer
50 mM Tris-HCl (pH 8.7).10 mM KCl.10 mM MgCl2.0.4 mg/ml bovine serum albumin.1.0 mM dithiothreitol.15% (v/v) glycerol.0.05 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP.10 μg activated DNA.Reaction volume of 50 μl.
Reaction Conditions
Incubation at 37 °C
100 Units;50 Units
20 mM Tris-HCl (pH 8.0)0.1 mM EDTA200 mM NaCl1 mM dithiothreitol50% (v/v) glycerol
Unit Definition
One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C under the stated assay conditions..

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