Human DNA Polymerase Beta

Name: Human DNA Polymerase Beta
SKU: EMDT0318

For research use only. Not intended for any clinical use.

Cat.No.

EMDT0318

Description

Exclusively from CHIMERx, Human DNA Polymerase Beta is a repair enzyme used to fill gaps and nicks in double-stranded DNA

Applications

Repair polymerase able to synthesize DNA beyond the end of a gap or nick with simultaneous displacement of the non-replicated strand.Fills gaps and nicks.

Reaction Buffer

50 mM Tris-HCl (pH 8.7).10 mM KCl.10 mM MgCl2.0.4 mg/ml bovine serum albumin.1.0 mM dithiothreitol.15% (v/v) glycerol.0.05 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP.10 μg activated DNA.Reaction volume of 50 μl.

Reaction Conditions

Incubation at 37 °C

Size/Form

100 Units;50 Units

Storage

20 mM Tris-HCl (pH 8.0)0.1 mM EDTA200 mM NaCl1 mM dithiothreitol50% (v/v) glycerol

Unit Definition

One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C under the stated assay conditions..

Publications

Q & A

Customer Reviews

Customer Q&As

What is the biological activity of Human DNA Polymerase Beta?

A: The enzyme activity of Human DNA Polymerase Beta is currently under testing, and we cannot guarantee it at the moment.

How is Human DNA Polymerase Beta obtained?

A: Human DNA Polymerase Beta is sourced from humans and is purified after expression in Escherichia coli.

What are the storage conditions for Human DNA Polymerase Beta?

A: It can be stored at -80°C for one year. To avoid repeated freeze-thaw cycles, it is recommended to aliquot and store upon receipt. After opening, it can be stored at -20°C for 3 months.

What precautions should be taken during use?

A: It should be noted that upon receipt, the product should be centrifuged before opening to prevent loss of freeze-dried powder.

What solvent should be used for reconstitution?

A: You can use a solution or culture medium containing a certain concentration of carrier protein (e.g., 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose) to reconstitute the product. When serum-free experiments are required, you can replace it with a 5% trehalose solution as a carrier.

Is the ED50 value on the product label accurate?

A: The ED50 value of recombinant protein products can vary with different batches or assay methods. It is recommended to determine the most suitable actual ED50 value for your experiments.

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