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Creative Biogene distinguishes itself by providing authentic and original pComb3 vectors optimized for high-throughput screening of protein interactions, selection of high-affinity binders, enzyme inhibitor screening, and ligand screening. The pComb3 phage display plasmid contains essential elements such as the f1 ori and gene sequences from M13 filamentous phage, enabling the production and screening of phages displaying proteins of interest. Additionally, the pComb8 variant offers multivalent expression capabilities, enhancing display efficiency through fusion with phage pVIII protein.
Key Features of Our Original pCombs
Our original pComb3 vector series is a classic tool in phage display, with a rich history and extensive literature support. Widely used for decades, including pComb3 and pComb3H, they provide a reliable platform for generating Fab antibody libraries with high success rates. While preserving the integrity of original designs and experimental protocols, we also offer improved and optimized variants like pComb3X and pComb3H for specific applications. Researchers can trust the stability, simplicity, and well-established performance of our original pComb3 vectors, playing an essential role in phage display research
Case Study 1
Researchers utilized the pComb8 vector to engineer M13 bacteriophages expressing MAGE-A1 tumor peptides, aiming to develop a melanoma vaccine. They tested the engineered bacteriophages in vivo to gauge their ability to stimulate immune responses. Two variants were engineered using phage display technology, each expressing MAGE-A1 peptides fused with different coat proteins. After intraperitoneal vaccination of mice, increased levels of anti-MAGE-A1 antibodies and enhanced cytotoxicity against melanoma cells were observed. These findings highlight the potential of engineered M13 bacteriophages for melanoma immunotherapy.
Figure 1. The successful construction of pComb8-MAGE and pComb3XSS-MAGE was confirmed by Sanger sequencing of the constructed phagemids. Genetically engineered M13 bacteriophages were then generated by transforming E. coli bacterial cells with the recombinant phagemids followed by super-infection with VCSM13 helper bacteriophages. (Brišar N, et al., 2024)
Case Study 2
Researchers aimed to identify conformational B-cell epitopes of the major timothy grass pollen allergen Phl p 1 using anti-idiotypic antibodies. They constructed a combinatorial phage display library from the IgG repertoire of a grass pollen-allergic patient, expecting to find anti-idiotypic Fab specificities. By biopanning with purified anti-Phl p 1 IgG, they isolated several Fab-displaying phage clones. Sequence analysis revealed four distinct clones, all derived from the same germline sequence, with high homology in their CDRs. Mapping their sequences onto the Phl p 1 structure revealed epitope patches on the N-terminal domain of the allergen, distinct from previously reported IgE epitopes. Thus, the study demonstrates that anti-idiotypic antibodies derived from the patient's IgG library, when used with the pComb3 vector, can mimic conformational epitopes of allergens, providing insights into their immunogenicity.
Figure. 2 The library was generated by integrating Fab-cDNA derived from PBMCs of an allergic donor into the XhoI/SpeI and SacI/XBaI sites of the pComb3 vector, enabling the fusion of the Fd chain with the C-terminal domain of the phage coat protein III (designated as library LB). (Lukschal A, et al., 2011)
Q: What makes Creative Biogene's The Original pCombs stand out from other phage display vectors on the market?
A: Creative Biogene's The Original pCombs are renowned for their authenticity, reliability, and consistent quality. Our phage display vectors are rigorously tested and validated to ensure optimal performance and reproducibility in a wide range of applications.
Q: How can I be sure that The Original pCombs will yield high-quality results in my research?
A: Creative Biogene prioritizes product quality and customer satisfaction. Our phage display vectors are manufactured using state-of-the-art technology and undergo stringent quality control measures to guarantee purity, stability, and functionality. Additionally, our experienced technical support team is available to assist with any inquiries or troubleshooting to ensure successful outcomes in your experiments.
Q: Can you provide examples of successful applications of The Original pCombs in research studies?
A: Numerous research studies have utilized The Original pCombs for antibody discovery, affinity maturation, protein engineering, and drug development projects. These vectors have been instrumental in identifying novel therapeutic targets, characterizing protein-protein interactions, and elucidating biological pathways across various fields of study.
Q: How does Creative Biogene ensure the authenticity and originality of The Original pCombs?
A: Creative Biogene takes pride in offering genuine and authentic phage display vectors that adhere to the highest standards of quality and integrity. Our products are meticulously designed and manufactured in-house by a team of skilled scientists and undergo rigorous testing to confirm their authenticity and efficacy.
Q: What sets Creative Biogene apart from other suppliers of phage display vectors?
A: Creative Biogene is committed to providing researchers with superior products, unparalleled customer service, and technical support. Our dedication to product excellence, innovation, and customer satisfaction distinguishes us as a trusted leader in the field of molecular biology and biotechnology.
| Cat.No. | Product Name | Price |
|---|---|---|
| VPT4010 | pComb3 vector | Inquiry |
| VPT4011 | pComb8 vector | Inquiry |
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