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The Signaling Pathway Reporter Cell Lines offered by Creative Biogene cover a wide range of cellular pathways and are available across various cell types. These cell lines are engineered to express luciferase or secreted alkaline phosphatase (SEAP) reporters under the control of specific pathway-responsive promoters. They serve as invaluable tools for studying diverse biological processes, including signal transduction, gene regulation, and cellular responses to external stimuli. The luciferase-based reporter cell lines enable real-time monitoring of pathway activation through luminescence assays, providing quantitative readouts of pathway activity. The SEAP-based reporter cell lines offer the advantage of secreted protein detection, allowing for non-destructive, kinetic monitoring of pathway activation in live cell cultures. With applications spanning from basic research to drug discovery and development, these Signaling Pathway Reporter Cell Line facilitate the investigation of various cellular pathways, including but not limited to the ATF6, AP1, AR, BMP, CHOP, CREB, ELK1, ER, HIF, IFN-α, IL6, IRF, NFAT, NF-kB, NRF2, p53, SMAD, SRF, STAT, and Wnt pathways. They can be utilized in studies involving pathway-specific modulation, drug screening, pathway crosstalk analysis, and disease modeling.
By leveraging Creative Biogene's Signaling Pathway Reporter Cell Lines, researchers gain access to reliable and efficient tools for elucidating the molecular mechanisms underlying cellular pathways, accelerating scientific discoveries, and advancing therapeutic interventions.
Key Features of Creative Biogene's Signaling Pathway Reporter Cell Lines
Signaling Pathway Reporter Cell Lines are sophisticated tools tailored to monitor specific signaling pathways within cells. Engineered with reporter genes downstream of responsive promoters, they enable precise detection upon pathway modulation. Categorized by targeted pathways like MAPK/ERK, PI3K/Akt, NF-κB, and Wnt/β-catenin, these lines facilitate:
Case Study 1
Inducing trained innate immunity by adjusting mature myeloid cells or their bone marrow precursors leads to a long-lasting heightened response to subsequent challenges. Researchers investigated whether inducing trained immunity via pre-treatment with β-glucan could enhance anti-tumor immunity. They found that β-glucan treatment led to reduced tumor growth in mice, associated with transcriptomic and epigenetic changes in granulopoiesis and neutrophil function. This effect was dependent on type I interferon signaling and could be transferred via adoptive transfer of neutrophils or bone marrow transplantation. These findings highlight a novel therapeutic approach harnessing trained immunity to modulate granulopoiesis for anti-tumor effects.
Figure 1. Researchers demonstrated the purpose of reporting cell line data to elucidate the impact of Type I IFN signaling on trained granulopoiesis' anti-tumor function. The B16-F10 melanoma cell line, which carries the luciferase reporter gene (B16-F10-Luc), was acquired from Creative Biogene. (Kalafati L, et al., 2020)
Case Study 2
NF-κB activation and inflammation are linked to autoimmune diseases, cancer, and neurological disorders like Alzheimer's. Researchers developed a stable neural NF-κB-reporter cell line to investigate NF-κB-driven neuroinflammation and assess drug efficacy. Using lentivirus, they transduced U251-MG glioma cells with a tandem NF-κB reporter construct. This allowed evaluation of NF-κB activity through various methods. Activation of NF-κB was observed upon exposure to proinflammatory molecules. The developed cell line proved effective in assessing both proand anti-inflammatory potential of pharmaceutical compounds, offering a valuable tool for studying neural inflammation and drug responses.
Figure 2. Researchers utilized cell lines to investigate endogenous TLR4 ligands' effects, validating responses through luciferase assays. (Zeuner MT, et al., 2017)
Q: What are the differences between Signaling Pathway Reporter Cell Lines and Constitutive Reporter Cell Lines?
A: The main difference between Signaling Pathway Reporter Cell Lines and Constitutive Reporter Cell Lines lies in their specific functionality. Signaling Pathway Reporter Cell Lines are engineered to respond to external stimuli or conditions by activating or inhibiting specific signaling pathways, thereby leading to the expression of reporter genes. In contrast, Constitutive Reporter Cell Lines constitutively express reporter genes without the need for external stimuli or pathway activation.
Q: How do Signaling Pathway Reporter Cell Lines help me understand the activity of a specific signaling pathway?
A: Signaling Pathway Reporter Cell Lines assist in elucidating the activity of specific signaling pathways by responding to external stimuli or conditions that activate or inhibit the pathway of interest. Upon pathway activation or inhibition, the reporter gene is expressed, allowing researchers to monitor and measure changes in pathway activity. This enables insights into the dynamics and regulation of signaling pathways in response to various stimuli or treatments.
Q: How should I choose a Signaling Pathway Reporter Cell Line suitable for my research purposes?
A: When selecting a Signaling Pathway Reporter Cell Line for research purposes, consider the specific signaling pathway you want to study and the stimuli or conditions relevant to your research. Ensure that the chosen cell line responds to the stimuli or conditions of interest and expresses reporter genes corresponding to the pathway being investigated. Additionally, assess the sensitivity and specificity of the reporter genes to ensure accurate detection of pathway activity under your experimental conditions.
Q: How stable are Signaling Pathway Reporter Cell Lines? What issues may arise in long-term experiments?
A: Signaling Pathway Reporter Cell Lines typically exhibit good stability, but some issues may arise inm long-term experiments. These could include changes in responsiveness to stimuli over time or alterations in reporter gene expression levels. To maintain stability, periodically validate the responsiveness and expression levels of reporter genes in the cell lines. Additionally, ensure consistency in experimental conditions to minimize variability and maintain reliable results over time.
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