Cat.No. : CSC-RR0589 Host Cell: MV4-11
| Cat. No. | CSC-RR0589 |
| Description | This cell line is engineered to stably co-express GFP and luciferase reporter genes in MV4-11 cells. |
| Gene | GFP/Luc |
| Host Cell | MV4-11 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Acute myeloid leukemia (AML) is a swiftly progressing and frequently lethal blood cancer, marked by the clonal expansion of leukemic stem cells (LSCs) and a differentiation blockade in the myeloid lineage, leading to the buildup of blasts. The researchers utilized GFP/Luc Reporter Cell Line - MV4-11 cells to investigate the activation of the GAS6/AXL axis in AML, focusing on its impact on stem/progenitor cells. They developed a potent AXL inhibitor with favorable pharmacological properties and efficacy in preclinical AML patient-derived xenotransplantation models. Their study demonstrated that AXL inhibition sensitizes AML stem/progenitor cells to venetoclax, showing strong synergistic effects both in vitro and in PDX models. Mechanistically, single-cell RNA-sequencing and functional validation revealed that AXL inhibition targets intrinsic metabolic vulnerabilities and alters the transcriptomic profile by inhibiting mitochondrial oxidative phosphorylation. Furthermore, inhibiting AXL or BCL-2 synergistically enhanced leukemic cell killing by targeting key signaling proteins, highlighting potential translational implications for AML and other AXL-active cancers.
Figure 1. The researchers injected MV4-11 cells carrying luciferase and GFP markers into NSG mice via an intravenous route. They treated mice with SLC-391, venetoclax, both, or vehicle orally for 3 weeks. Bioluminescent imaging was conducted to quantify signal intensity, revealing significantly reduced signals in SLC-391 and combination-treated groups compared to controls. (Niu X et al., 2021)
A: GFP and luciferase in the MV4-11 cell line are usually co-expressed under the same expression vector, or by inserting into different sites respectively. This dual reporter gene system allows researchers to simultaneously monitor two independent biological processes within cells. For example, GFP can be used to observe changes in cell morphology and position in real time, while luciferase can be used to quantitatively analyze the activity of specific signaling pathways.
A: The MV4-11 cell line is a human acute myeloid leukemia (AML) cell line with broad experimental application prospects. The design of the dual reporter gene system gives it obvious advantages in high-throughput screening, signaling pathway analysis and gene function research. By simultaneously detecting GFP and luciferase signals, more comprehensive experimental data can be obtained, such as evaluating the comprehensive effects of drugs on cell proliferation, migration, and specific signaling pathways.
A: GFP and luciferase reporter gene expression in the MV4-11 cell line are highly stable. This is due to the optimized gene integration strategy and the use of strong promoters, which ensures the continuous expression of the reporter gene during cell division and passage. In experiments, the stability of the reporter gene allows researchers to conduct dynamic monitoring over a long period of time, providing reliable and reproducible experimental results.
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The product arrived quickly and on time, with the packaging intact. When I opened the package, I immediately noticed that the packaging was meticulous and that appropriate measures were taken to protect the integrity of the cell line.
The gene expression of the cell line is very stable, and the expression of GFP and Luciferase can be accurately monitored. This provides reliable data support for my research and allows me to explore the pathogenesis of leukemia more deeply.
The MV4-11 cell line provides an ideal model system to help me study the mechanisms of leukemia and the effects of drug treatment. Real-time monitoring of gene expression and cell activity is more convenient and saves a lot of time.
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