Cat.No. : CSC-RR0484 Host Cell: JeKo-1
| Cat. No. | CSC-RR0484 |
| Description | GFP/Luc Reporter Cell Line - Jeko-1 is engineered to stably express GFP and luciferase encoding genes. This cell line is an ideal tool for fluorescent and bioluminescent tracing of Jeko-1 cells. JeKo-1 is a mantle cell lymphoma (MCL) cell line which is derived from peripheral blood mononuclear cells. |
| Gene | GFP/Luc |
| Host Cell | JeKo-1 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
BAFF, or B cell-activating factor, primarily binds to three receptors: BAFF-R, BCMA, and TACI, which are predominantly found on mature B cells. Researchers developed a BAFF ligand-based chimeric antigen receptor (CAR) and generated BAFF CAR-T cells using non-viral gene delivery. These BAFF CAR-T cells specifically bind to BAFF receptors BAFF-R, BCMA, and TACI, prevalent on mature B cells. In vitro and in vivo studies demonstrate their efficacy in killing various B cell cancers, including mantle cell lymphoma, multiple myeloma, and acute lymphoblastic leukemia. Co-culture with tumor cells induces activation marker CD69, degranulation marker CD107a, and proinflammatory cytokine production. This ligand-based BAFF CAR-T strategy targets multiple receptors, reducing the risk of antigen escape in B cell cancer treatment.
Figure 1. BAFF CAR-T cell function and specificity were characterized and validated using Luc Reporter Cell Line - Jeko-1. Luminescence was measured after co-culturing with luciferase-expressing Jeko-1 cells. Cytotoxicity was assessed by PI staining after co-culturing with HEK293T cells expressing BAFF-R, BCMA, or TACI. Adverse toxicity potential was evaluated by LDH release after co-culturing with primary human cells. (Wong DP, et al., 2022)
A: The GFP/Luc Reporter Cell Line in Jeko-1 cells offers a reliable platform for studying gene expression and cellular responses in lymphoma research. Jeko-1 cells are a well-established model for B-cell lymphoma, providing a relevant background for investigating lymphoma biology. The dual-reporter system with GFP and luciferase allows for comprehensive analysis of cellular processes and treatment responses.
A: The stability and consistency of GFP and luciferase expression in the Jeko-1 reporter cell line are ensured through stringent quality control measures. Stable transfection techniques are utilized to integrate the reporter genes into the Jeko-1 cell genome, followed by clonal selection to isolate cell lines with stable expression levels. Regular monitoring and validation of reporter expression ensure reliable and reproducible results in experiments.
A: Yes, the GFP/Luc Reporter Cell Line in Jeko-1 cells can be customized to incorporate specific regulatory elements or pathways relevant to your research interests. Our team can tailor the cell line to match your experimental requirements, providing a personalized solution for studying lymphoma biology or investigating specific signaling pathways.
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Consistent performance! The GFP/Luc Reporter Cell Line consistently delivers bright signals, ensuring precise monitoring and quantification of cellular events in vitro and in vivo. Its dual-reporter system simplifies data collection and interpretation, accelerating discoveries in lymphoma research and drug development.
From studying tumor-immune interactions to evaluating treatment efficacy, this cell line serves as a valuable resource for elucidating lymphoma biology and identifying novel therapeutic targets.
With both reporters, this cell line enables detailed investigation of tumor cell behavior, microenvironment interactions, and therapeutic responses, advancing our understanding of lymphoma pathogenesis. The GFP/Luc Reporter Cell Line in Jeko-1 cells combines GFP fluorescence and luciferase bioluminescence, offering comprehensive insights into lymphoma biology.
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