Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR00983
Host Cell : RT4 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR00983 |
| Description | RT4-GFP/Luc cell line is engineered to co-express GFP and luciferase reporter genes in RT4 cells. GFP and luciferase are useful biomarkers and are widely used in cell research to label and monitor various types of cells. This cell line is a powerful tool in both fluorescent and bioluminescent tracking of RT4 cells. |
| Product Type | Stable cell line expressing GFP and luciferase reporter genes |
| Target Gene | GFP/Luc |
| Host Cell | RT4 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1) in vitro cell tracking by both the fluorescent and bioluminescent signal 2) monitor in vivo tumor growth using both the fluorescence and bioluminescence signal 3) anticancer drug development |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) fluorescence detection under microscopy 2) in vitro cell luciferase assay 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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We transitioned to this RT4 reporter line for our bladder cancer xenograft program and couldn’t be happier. The cells are remarkably easy to culture and maintain their reporter expression even after prolonged passaging. The GFP signal is bright enough for FACS-based sorting without additional staining, and the luciferase readout is highly sensitive.
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