Pages
Products

GFP/Luc Reporter Cell Line - A-673

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR00988

Host Cell :   A-673 Size :   >1x106 frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RR00988
Description A-673-GFP/Luc cell line is engineered to co-express GFP and luciferase reporter genes in A-673 cells. GFP and luciferase are useful biomarkers and are widely used in cell research to label and monitor various types of cells. This cell line is a powerful tool in both fluorescent and bioluminescent tracking of A-673 cells.
Product Type Stable cell line expressing GFP and luciferase reporter genes
Target Gene GFP/Luc
Host Cell A-673
Host Cell Species Homo sapiens (Human)
Applications 1) in vitro cell tracking by both the fluorescent and bioluminescent signal
2) monitor in vivo tumor growth using both the fluorescence and bioluminescence signal
3) anticancer drug development
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) fluorescence detection under microscopy
2) in vitro cell luciferase assay
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Great value for the price

The dual GFP/Luc reporters are expressed at high levels, with the luciferase signal showing an excellent linear correlation with cell number. We have used this line successfully in both flank and metastatic models. The in vivo bioluminescence imaging results are clean and highly reproducible.

Japan

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction