Transfected Stable Cell Lines
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Cat. No. : CSC-RR0567M
Host Cell : U266 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0567M |
| Description | U266-GFP/Luc cell line is engineered to stably overexpress turboGFP and Firefly luciferase reporter genes under the control of the CMV promoter. |
| Target Gene | GFP/Luc |
| Host Cell | U266 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter Type | Fluorescent protein |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
U266 cells are a human multiple myeloma-derived cell line widely used in research due to their indefinite proliferative capacity and their ability to recapitulate key features of plasma cell malignancies.
The U266-GFP/Luc cell line was engineered by stably integrating green fluorescent protein (GFP) and luciferase (Luc) reporter genes into U266 cells. The GFP reporter enables real-time visualization and tracking of cells in both live animal models and in vitro experiments using fluorescence microscopy. Meanwhile, the luciferase reporter allows for quantitative assessment of cell viability and tumor growth via bioluminescence imaging, which detects light emission from the enzymatic reaction between luciferase and its substrate.
This dual-reporter system provides researchers with a powerful tool to:
By delivering quantitative data on tumor burden and cellular behavior, the U266-GFP/Luc cell line serves as an essential model for advancing our understanding of multiple myeloma and developing more effective treatments.
The GFP/Luc reporter cell line - U266 has five applications:
Drug screening: The GFP/Luc reporter cell line can be used to evaluate the efficacy and cytotoxicity of new drug candidates by monitoring GFP and luciferase activity, allowing researchers to efficiently identify potential therapeutic agents.
Gene expression studies: Researchers can use this cell line to study gene expression regulation and functional analysis of target genes, using dual reporters for both quantitative and qualitative assessments.
Signal transduction studies: The GFP/Luc reporter cell line is a valuable tool for examining intracellular signal transduction pathways. The dual reporter provides real-time insight into the activation or inhibition of specific signaling cascades.
Cancer research: The GFP/Luc reporter cell line is particularly useful in multiple myeloma research, facilitating the study of cancer cell behavior, including proliferation, apoptosis, and metastasis, under a variety of experimental conditions.
In vivo imaging: The luciferase reporter enables non-invasive, real-time imaging of tumor growth and metastasis in animal models, enhancing the understanding of tumor dynamics and the effectiveness of anticancer treatments.
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As a long-term user of various reporter cell lines, I can confidently say that the GFP/Luc reporter cell line - U266 stands out. Great product, highly recommended!
GFP/Luc Reporter Cell Line - U266 exceeded our expectations in terms of ease of use and reliability. The dual reporter is very effective for tracking and analyzing gene expression.
I am very impressed with the GFP/Luc Reporter Cell Line - U266. The dual reporter system (GFP and luciferase) is very powerful and provides consistent and reliable results in my gene expression studies. It is an invaluable tool in my research.
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