GFP/Luc Reporter Cell Line - ECA109

ECA109 is an adherent human cancer cell line associated with esophageal squamous cell carcinoma and has been used extensively in experimental studies of esophageal tumor biology. The cells display epithelial growth characteristics and can form tumors in immunodeficient mice under appropriate experimental conditions. GFP/Luc Reporter Cell Line - ECA109 is an engineered derivative expressing green fluorescent protein and firefly luciferase. GFP enables direct visualization of reporter-positive cells by fluorescence microscopy or flow cytometry, while luciferase produces bioluminescence after exposure to a compatible luciferin substrate. These complementary signals allow researchers to identify the cells visually and obtain a sensitive quantitative measurement from the same model.

The dual-reporter line is suitable for studying esophageal cancer cell proliferation, survival, migration, invasion, and treatment response. GFP fluorescence can be used to examine cell morphology, localization, and interactions with stromal or immune cells in two-dimensional cultures, spheroids, and other co-culture systems. Luciferase provides a convenient readout for viability and cytotoxicity assays, supporting compound screening and comparisons of chemotherapy, targeted agents, biologics, or combination treatments. In xenograft studies, bioluminescence imaging may allow repeated, non-invasive monitoring of tumor establishment, growth, dissemination, and therapeutic response in the same animal. GFP can complement these measurements by supporting ex vivo imaging and identification of reporter-positive cells in excised tissues. Together, the two reporters make this line useful for mechanistic studies, drug-response evaluation, and preclinical imaging research involving esophageal squamous cell carcinoma.