GFP/Luc Reporter Cell Line - U-87MG

Name: GFP/Luc Reporter Cell Line - U-87MG
SKU: CSC-RR00974

Cat.No. : CSC-RR00974 Host Cell: U-87MG

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Cat. No.	CSC-RR00974
Description	U-87MG-GFP/Luc cell line is engineered to co-express GFP and luciferase reporter genes in U-87MG cells. GFP and luciferase are useful biomarkers and are widely used in cell research to label and monitor various types of cells. This cell line is a powerful tool in both fluorescent and bioluminescent tracking of U-87MG cells.
Gene	GFP/Luc
Host Cell	U-87MG
Host Cell Species	Homo sapiens (Human)
Stability	This cell line is stable at least 10 passages.
Product Type	Stable cell line expressing GFP and luciferase reporter genes
Applications	1) in vitro cell tracking by both the fluorescent and bioluminescent signal 2) monitor in vivo tumor growth using both the fluorescence and bioluminescence signal 3) anticancer drug development
Quality Control	1) fluorescence detection under microscopy 2) in vitro cell luciferase assay 3) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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The researchers developed a three-dimensional in vitro glioblastoma (GBM) model integrating U-87 MG spheroids, hyaluronan (HA)-loaded alginate (Alg) hydrogels, and a blood–brain barrier (BBB) component to investigate the influence of HA molecular weight on tumor invasion and barrier permeability. U-87 MG (GFP+) cells obtained from Creative Biogene were expanded under standard culture conditions and assembled into spheroids, which were subsequently encapsulated within Alg hydrogels containing HA of distinct molecular weights to mimic key features of the GBM tumor microenvironment. This platform enabled systematic evaluation of cancer cell migration dynamics, invasive phenotype, and BBB/BBTB permeability in response to extracellular matrix composition.

Figure 1. Migration assays demonstrate differential invasion of U-87 spheroids encapsulated in Alg hydrogels containing HA of varying molecular weights, as quantified over time and visualized by optical and fluorescence microscopy. (Andrade F, et al., 2026)

Creative Biogene–derived U-87 MG cells provide a robust and reproducible foundation for advanced 3D tumor modeling studies. Their compatibility with biomaterial-based spheroid systems supports mechanistic investigations into tumor microenvironment–driven invasion and barrier dysfunction in glioblastoma research.

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