GFP/Luc Reporter Cell Line - U-87MG

The researchers developed a three-dimensional in vitro glioblastoma (GBM) model integrating U-87 MG spheroids, hyaluronan (HA)-loaded alginate (Alg) hydrogels, and a blood–brain barrier (BBB) component to investigate the influence of HA molecular weight on tumor invasion and barrier permeability. U-87 MG (GFP+) cells obtained from Creative Biogene were expanded under standard culture conditions and assembled into spheroids, which were subsequently encapsulated within Alg hydrogels containing HA of distinct molecular weights to mimic key features of the GBM tumor microenvironment. This platform enabled systematic evaluation of cancer cell migration dynamics, invasive phenotype, and BBB/BBTB permeability in response to extracellular matrix composition.

Figure 1. Migration assays demonstrate differential invasion of U-87 spheroids encapsulated in Alg hydrogels containing HA of varying molecular weights, as quantified over time and visualized by optical and fluorescence microscopy. (Andrade F, et al., 2026)

Creative Biogene–derived U-87 MG cells provide a robust and reproducible foundation for advanced 3D tumor modeling studies. Their compatibility with biomaterial-based spheroid systems supports mechanistic investigations into tumor microenvironment–driven invasion and barrier dysfunction in glioblastoma research.