EF1α-GFP-2A-FLuc-IRES-Puro Lentivirus

Name: EF1α-GFP-2A-FLuc-IRES-Puro Lentivirus
SKU: LV00997Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV00997Z

Storage : -80℃ Shipping : Frozen on dry ice

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Virus Particles Information

Quality Control

Cat. No.	LV00997Z
Description	This lentivirus contains 2A linked GFP and Firefly luciferase reporter gene as well as IRES-mediated puromycin resistance gene under EF1a promoter.
Gene	GFP/Luc
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

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Lentivirus vector (LV) belongs to the family Retrovidae and is an RNA virus. Different from general retrovirus vectors, it has the ability to infect both dividing and non-dividing cells. This vector can effectively integrate exogenous genes into host chromosomes, thereby achieving persistent expression. In terms of infection ability, it can effectively infect various types of cells such as neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, etc., thereby achieving good gene therapy effects. Clinical studies have been carried out and the results are very ideal, so it has broad application prospects.

Compared with other viral tools, lentivirus has the following advantages:

1) Long expression time: Lentivirus can achieve long-term and stable expression of the target gene by integrating exogenous genes into the host cell genome. It is not lost with cell division and generation, and is the first choice for cell experiments;

2) High safety: No pathogenicity has been found, and it has been used for CAR-T therapy in humans;

3) Low immunogenicity: Direct injection of living tissue is not easy to cause immune response, and is suitable for animal experiments.

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Customer Reviews

Bright & Consistent Luciferase Signal

The FLuc reporter produced bright luminescence in vivo, perfect for our tumor xenograft model. Data reproducibility was outstanding.

French

2023-02-11

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EF1α-GFP-2A-FLuc-IRES-Puro Lentivirus

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Bright & Consistent Luciferase Signal

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