GFP/Luc Reporter Cell Line - HCC1937

HCC1937 is a human breast ductal carcinoma cell line derived from a primary breast tumor of the basal-like/triple-negative subtype. It is characterized by deficiency in BRCA1 function and is widely used as a model system for studying homologous recombination repair defects, genomic instability, and aggressive breast cancer biology. Due to its triple-negative phenotype (ER-, PR-, HER2-), HCC1937 cells are particularly relevant for investigating treatment-resistant breast cancer subtypes and DNA damage response pathways. The GFP/Luc Reporter Cell Line - HCC1937 is established through stable transfection to co-express green fluorescent protein (GFP) and firefly luciferase (Luc) reporter genes in HCC1937 cells. This dual-reporter design enables both real-time fluorescence imaging and quantitative bioluminescence detection, while preserving the parental genetic and phenotypic characteristics of the original cell line.

The HCC1937-GFP/Luc reporter cell line is widely used in breast cancer research, particularly in xenograft models for monitoring tumor initiation, progression, and metastatic behavior in vivo. The luciferase signal provides a sensitive and non-invasive method for longitudinal tracking of tumor burden and therapeutic response, while GFP expression allows direct visualization of cellular distribution and morphology in vitro and in harvested tissues. This model is especially valuable for evaluating the efficacy of chemotherapeutic agents and targeted therapies, including DNA damage response inhibitors such as PARP inhibitors, in BRCA1-deficient tumor contexts. In addition, it is applied in mechanistic studies of tumor microenvironment interactions, clonal evolution, and gene regulation under genotoxic stress. Overall, the HCC1937-GFP/Luc cell line offers a robust and reproducible platform for preclinical breast cancer research, particularly for studying aggressive and treatment-resistant triple-negative breast cancer.