GFP/Luc Reporter Cell Line - MHCC97H

MHCC97H is a human hepatocellular carcinoma (HCC) cell line established from a metastatic liver cancer lesion and is well recognized for its high metastatic potential. Derived from the MHCC97 series, MHCC97H exhibits aggressive growth characteristics and a strong capacity for invasion and distant metastasis, making it a valuable model for studying advanced hepatocellular carcinoma and metastatic progression. The cell line retains key molecular and phenotypic features of human HCC and is frequently employed in investigations of tumor dissemination, angiogenesis, and therapeutic resistance. The GFP/Luc Reporter Cell Line - MHCC97H is generated through stable transduction of green fluorescent protein (GFP) and firefly luciferase (Luc) reporter genes into MHCC97H cells. This dual-reporter system enables both fluorescence-based visualization and highly sensitive bioluminescence detection while maintaining the metastatic behavior and tumorigenic properties of the parental cell line.

The MHCC97H-GFP/Luc reporter cell line is widely used in liver cancer research, particularly for establishing orthotopic and metastatic xenograft models that closely mimic the progression of advanced hepatocellular carcinoma. Luciferase expression enables non-invasive, real-time monitoring of primary tumor growth and metastatic spread through bioluminescence imaging, allowing longitudinal assessment of disease progression in living animals. GFP expression facilitates visualization of tumor cell localization, invasion patterns, and metastatic colonization in cultured cells and tissue specimens. This model is especially valuable for evaluating anti-cancer agents targeting tumor growth, angiogenesis, invasion, and metastasis. In addition, it supports mechanistic studies of epithelial–mesenchymal transition, tumor microenvironment interactions, cancer stem cell biology, and signaling pathways involved in metastatic dissemination. Owing to its stable reporter expression and highly metastatic phenotype, the MHCC97H-GFP/Luc cell line serves as a robust and reproducible platform for preclinical drug evaluation and translational research in hepatocellular carcinoma.