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GFP/Luc Reporter Cell Line - TE-10

GFP/Luc Reporter Cell Line - TE-10

Cat.No. :  CSC-RR0537 Host Cell:  TE-10

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Cat. No. CSC-RR0537
Description This cell line is engineered to stably overexpress GFP and luciferase reporter genes. TE-10 is a human esophageal squamous cancer cell line. This cell line is a useful tool for both fluorescent and bioluminescent tracking of TE-10 cells.
Gene GFP/Luc
Host Cell TE-10
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Reporter Type Fluorescent protein
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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The GFP gene produces a fluorescent protein that can be detected using a fluorescence microscope or a flow cytometer. It is a popular tool for visualizing gene expression and protein localization in living cells. On the other hand, the Luc gene encodes the luciferase enzyme, which catalyzes the conversion of luciferin into oxyluciferin, resulting in the production of light. This bioluminescence signal can be detected using a luminometer or a photon-counting camera. TE-10 Cell Line is a human epithelial ovarian cancer cell line that has been used extensively in ovarian cancer research. It was established from a patient with epithelial ovarian cancer and has become an important model for studying the pathogenesis and treatment of the disease. Some of the major advantages of using TE-10 cells include rapid growth, ease of culture, and karyotypic stability. These cells also exhibit a high degree of tumourigenicity, making them suitable for preclinical studies of cancer treatment. In addition, TE-10 cells express a wide range of cell surface antigens, making them suitable for immune-based studies. In conclusion, the TE-10 cell line is an important tool for understanding and treating epithelial ovarian cancer.
The GFP/Luc Reporter TE-10 Cell Line is a dual reporter system that combines the benefits of green fluorescent protein (GFP) and luciferase assays to provide a powerful tool for gene expression and regulation studies in ovarian cancer research. The cell line is derived from the human epithelial ovarian cancer cell line TE-10 and expresses both GFP and luciferase under the control of the relevant promoters. One of the key advantages of using the GFP/Luc reporter TE-10 cell line, is the ability to simultaneously monitor the expression and activity of target genes or proteins, with GFP allowing visualisation of cellular localisation and protein distribution, and luciferase providing quantitative measurements of gene expression levels. This dual-reporting system allows researchers to study the dynamics of gene regulation and protein function more comprehensively. The cells are easy to culture and grow relatively quickly, providing researchers with a stable and reliable experimental system. The stable karyotype of the cell line ensures long-term consistency of experimental results. The GFP/Luc reporter gene TE-10 Cell Line can be used for a variety of applications, such as studying the effects of different drugs or chemicals on gene expression, investigating the function of specific transcription factors, or studying the regulatory mechanisms of various biological processes in ovarian cancer. Its versatility and ease of use make it an essential tool for researchers in the field of ovarian cancer research and an invaluable resource for advancing our understanding of gene expression and regulation in this disease.
Customer Q&As
What is TE-10 Cell Line?

A: TE-10 Cell Line is a human epithelial cell line derived from the thyroid carcinoma cell line TEC. It is often used in research to study the behavior and characteristics of thyroid epithelial cells, providing a valuable model for investigating thyroid cancer and related diseases.

What are the characteristics of TE-10 Cell Line?

A: TE-10 Cell Line exhibits typical characteristics of thyroid epithelial cells, including the expression of thyroid-specific markers such as thyroid peroxidase (TPO) and thyroglobulin (Tg). It also displays a relatively high degree of proliferation and invasive potential, which makes it suitable for studying the aggressive behavior of thyroid cancer cells.

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