CMV-FLuc-EF1a-GFP Lentivirus

The CMV-FLuc-EF1a-GFP lentiviral vector is a powerful viral vector system that combines the advantages of dual reporter genes with lentiviral transduction. This genetically engineered lentiviral vector contains two independent expression cassettes: one encoding the firefly luciferase (FLuc) reporter gene driven by a strong constitutive CMV promoter, and the other encoding the GFP reporter gene controlled by the human EF1α promoter. The lentiviral platform offers several key advantages, including stable integration into the host genome, the ability to infect both dividing and non-dividing cells, and high transduction efficiency across a wide range of cell types. The dual reporter gene design allows researchers to simultaneously monitor transduced cells using bioluminescence imaging (FLuc) and fluorescence detection (GFP), providing multiple tracking capabilities. Other advantages include high viral titers achievable through concentration methods, a broad host range, and the safety of a third-generation packaging system employing a deletion of viral accessory genes.

This lentiviral vector has broad applications in biomedical research and drug development. The FLuc reporter gene allows for long-term monitoring of cell populations in vivo using bioluminescence imaging, making it ideal for tumor xenograft studies, stem cell tracking, and metastasis model research. The EF1α promoter-driven GFP provides a convenient fluorescent marker for cell sorting, microscopy analysis, and in vitro cell tracking. Researchers frequently use this system to generate stable cell lines, conduct gene function studies, and create transgenic animal models. The combination of optical reporter genes enables correlation between in vivo imaging data and in vitro fluorescence validation. Other applications include lentiviral library screening, where GFP helps assess transduction efficiency, while FLuc provides a functional readout.