Cat.No. : CSC-RR0492 Host Cell: HepG2
| Cat. No. | CSC-RR0492 |
| Description | HepG2/GFP-Luc is derived from HepG2 cell line which has been transduced by two lentiviruses, one containing EGFP and puromycin resistance gene, the other containing Firefly luciferase and neomycin resistance gene. HepG2 cell line has been widely used in liver cancer research. This cell line is an ideal tool to support research in both fluorescent and bioluminescent tracing of HepG2 cells. |
| Gene | GFP/Luc |
| Host Cell | HepG2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Oncolytic adenoviruses, particularly type 5 (Ad5), have emerged as promising agents in cancer therapy. However, their effectiveness is often hampered by the presence of neutralizing antibodies and reduced receptor expression on many tumor types. Recent studies have explored alternative adenovirus types, specifically species D, which have shown enhanced transduction and cytotoxicity in various cancer cell lines, including HepG2. Researchers evaluated the oncolytic potential of replication-competent species D adenoviruses engineered to express a GFP-Luciferase reporter, revealing that these vectors could effectively transduce HepG2 cells. This ability to deliver therapeutic genes makes them a valuable tool for studying gene expression and cellular responses in cancer research.
Figure 1. The researchers infected cells with 500vp/cell of Ad-GFP-Luc viruses, analyzing GFP+ cells 24 hours later using flow cytometry for transduction assessment. (Bullard BL, et al., 2020)
Creative Biogene's GFP/Luc Reporter Cell Line - HepG2 provides a robust platform for researchers interested in exploring gene expression and the efficacy of oncolytic therapies. This cell line can facilitate studies of viral transduction efficiency and gene expression modulation in hepatocellular carcinoma models, mirroring the functionality demonstrated in recent adenoviral research.
A: The GFP/Luc Reporter Cell Line - HepG2 can be utilized in various applications for studying hepatocellular carcinoma progression. Firstly, it allows for the visualization of HepG2 cells in vitro and in vivo through GFP fluorescence, aiding in tracking tumor growth and metastasis. Secondly, the luciferase reporter enables quantitative assessment of tumor progression and response to treatments using bioluminescence assays.
A: In drug screening studies targeting liver cancer, the GFP/Luc Reporter Cell Line - HepG2 provides a valuable tool for evaluating the efficacy of potential therapeutics. Researchers can monitor changes in GFP fluorescence and luciferase activity as indicators of drug response, allowing for high-throughput screening of candidate compounds and identification of promising leads for further investigation.
A: The GFP/Luc Reporter Cell Line - HepG2 offers several advantages over conventional cell lines in studying liver biology. Firstly, its stable expression of GFP and luciferase allows for real-time monitoring of cellular processes and responses to stimuli. Secondly, the HepG2 cell line, derived from hepatocellular carcinoma, retains characteristics of liver cells, making it a relevant model for studying liver diseases and drug metabolism.
A: By utilizing the GFP/Luc Reporter Cell Line - HepG2 in co-culture experiments with stromal cells or immune cells, researchers can elucidate the influence of the tumor microenvironment on hepatocellular carcinoma progression. The GFP fluorescence allows for tracking of HepG2 cells, while luciferase activity enables quantification of changes in tumor growth and response to microenvironmental factors or therapeutic interventions.
A: Establishing stable cell lines with the GFP/Luc Reporter Cell Line - HepG2 involves transfecting HepG2 cells with plasmids containing the GFP and luciferase genes, followed by selection with antibiotics or selection markers. Single-cell cloning can be performed to isolate individual clones with stable expression of both reporters. These clones can then be expanded and characterized for their GFP fluorescence and luciferase activity prior to experimental use.
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The GFP/Luc Reporter Cell Line - HepG2 allows simultaneous visualization and quantification of cellular events, providing a dual-reporter system that enhances the depth and breadth of biological assays.
HepG2 cells are derived from liver tissue, making the GFP/Luc Reporter Cell Line - HepG2 particularly relevant for liver-related research, such as metabolism studies and hepatotoxicity assays.
GFP provides immediate visual confirmation of transfection efficiency and gene expression, streamlining the workflow for the GFP/Luc Reporter Cell Line - HepG2 and reducing the time to results.
The luciferase aspect of the GFP/Luc Reporter Cell Line - HepG2 offers highly sensitive bioluminescence quantification, which is ideal for detecting and measuring promoter activity and gene regulation.
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