GFP/Luc Reporter Cell Line - SIHA

SiHa is a human cervical squamous cell carcinoma cell line derived from a moderately differentiated invasive carcinoma of the uterine cervix. It is characterized by integration of human papillomavirus type 16 (HPV16) DNA and is widely used as an in vitro model for studying cervical cancer biology, tumor progression, and HPV-associated oncogenesis. The GFP/Luc Reporter Cell Line - SiHa is generated by stable genetic modification to co-express green fluorescent protein (GFP) and firefly luciferase (Luc). This dual-reporter system enables real-time monitoring of cellular behavior through fluorescence microscopy and quantitative bioluminescence imaging. The modified cells retain the growth characteristics of the parental SiHa line while providing enhanced sensitivity for dynamic tracking in both in vitro and in vivo experimental settings.

The SiHa-GFP/Luc reporter cell line is widely applied in cancer research, particularly in the establishment of tumor xenograft models for evaluating tumor growth and metastatic potential in vivo. The luciferase signal allows non-invasive, longitudinal monitoring of tumor burden in living animals through bioluminescence imaging, while GFP expression facilitates fluorescence-based visualization of cellular morphology and localization in vitro and in excised tissues. This model is also valuable for high-throughput drug screening and therapeutic efficacy assessment, enabling rapid quantification of treatment response over time. In addition, the dual-reporter system supports studies on gene regulation, cell proliferation, and migration dynamics under different experimental conditions. Its stable and consistent signal output improves reproducibility in longitudinal experiments, making it a reliable tool for preclinical evaluation of anti-cancer agents and for investigating mechanisms underlying cervical cancer progression and metastasis.