Cat.No. : CSC-RR0374 Host Cell: K562
| Cat. No. | CSC-RR0374 |
| Description | This cell line is engineered to stably express GFP and luciferase repoter genes. It is a useful tool for both fluorescent and bioluminescent tracking of K562 cells. |
| Gene | GFP/Luc |
| Host Cell | K562 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
ASXL1 mutations are found in all myeloid neoplasms, including CMML, and are linked to worse outcomes. Researchers aimed to clarify the role of RUNX1 mutations (RUNX1-MT) in ASXL1-mutated leukemia. Co-expression of RUNX1-MT (R135T) and ASXL1-MT (R693X) in cell lines was followed by various in vitro and in vivo analyses. Results showed that combined mutations enhanced myeloid stem cell expansion, promoting leukemia development. RUNX1-MT expression in ASXL1-mutated myeloid cells increased proliferation, blocked differentiation, and boosted self-renewal. Mice with both mutations displayed characteristic disease features. Notably, RUNX1-MT upregulated inhibitor of DNA binding 1 (ID1) expression in multiple tissues, suggesting its involvement in leukemia development. These findings highlight the crucial role of RUNX1-MT in ASXL1-mutated leukemia pathogenesis, providing insights into myeloid malignancies.
Figure 1. Researchers transfected K562 cells with luciferase reporter plasmid under HRE promoter to assess RUNX1 and ASXL1 mutant effects. They incubated cells with CoCl2 to mimic hypoxic conditions and evaluated luciferase activities. Relative luciferase intensity normalized to GFP and EV control was measured after 72 hours. (Bera R, et al., 2019)
A: Stable integration of luciferase and GFP reporter genes into the K562 cell genome was ensured through robust transfection techniques. Clonal selection isolated cell lines with stable reporter expression. Regular monitoring using assays like luciferase activity and fluorescence microscopy maintained consistent expression levels.
A: The Luc/GFP Reporter Cell Line - K562 allows for the investigation of diverse cellular processes, including gene expression regulation, signal transduction pathways, and drug response mechanisms. The dual-reporter system facilitates real-time monitoring and visualization of these processes in leukemia cells.
A: The Luc/GFP Reporter Cell Line - K562 is suitable for high-throughput screening assays in leukemia research. The combination of luciferase and GFP reporters enables efficient detection and quantification of cellular responses to various stimuli or drug treatments, making it ideal for large-scale screening studies.
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From studying leukemia progression to evaluating drug responses, this cell line serves as a valuable tool for advancing our understanding of leukemia biology and identifying novel treatment strategies.
The Luc/GFP Reporter Cell Line consistently delivers bright signals, ensuring precise monitoring and quantification of cellular events in vitro and in vivo. Its dual-reporter system simplifies data collection and interpretation, accelerating discoveries in leukemia pathogenesis and therapeutic development.
The Luc/GFP Reporter Cell Line in K562 cells illuminates research with luciferase bioluminescence and GFP fluorescence, enabling multifaceted insights into leukemia biology. Dual fluorescence brilliance!
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