Cat.No. : CSC-RR0542 Host Cell: Jurkat
| Cat. No. | CSC-RR0542 |
| Description | This cell line is engineered to stably overexpress GFP and luciferase reporter genes. Jurkat is an immortalized human T lymphocyte cells and has been widely used in studying acute T cell leukemia, T cell signaling etc. This cell line is a useful tool for both fluorescent and bioluminescent tracking of Jurkat cells. |
| Gene | GFP/Luc |
| Host Cell | Jurkat |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Human immunodeficiency virus type 1 (HIV-1) is the pathogen that causes acquired immunodeficiency syndrome (AIDS). Since its discovery in 1981, it has caused approximately 40.1 million deaths (UNAIDS, 2023). Researchers used the GFP/Luc Reporter Cell Line - Jurkat cell line to characterize the interaction between extracellular particles (EPs) and human immunodeficiency virus type 1 (HIV-1). Five different grades (Frac-A to Frac-E) of particles were isolated from HIV-infected cells by sequential differential ultracentrifugation (DUC). The results showed that the median particle size was greater than 100 nm for most of the grades, but the average particle size was much smaller than 50 nm for Frac-E. Using these cell lines, the researchers detected that Frac-A contained large infectious particles, while Frac-E contained particles harboring CD63, HSP70, and HIV-1 proteins in small particles. Despite the smaller average size of Frac-E particles, the viral integrase in them was only detectable by SDS treatment, suggesting that they were encapsulated within vesicles. Single-particle analysis further confirmed that CD63, HIV-1 integrase, and the envelope glycoprotein Env co-localized to the same Frac-E particle. Notably, Frac-E particles were infectious and their infectivity was significantly reduced after immunobreaking with anti-CD63. These findings suggest for the first time that small particles of HIV-1 may break through the currently accepted range of their size and may have more profound implications for viral pathogenicity.
Figure 1. The researchers utilized a GFP/Luc reporter cell line, the Jurkat cell line, to assess the functional activity of HIV-1 viral particles in EPs. Different EPs fractions were first added to MOLT-4 target cells for VRA experiments, and viral p24 antigen was detected in the supernatant on day 4. The supernatants were then transferred to A3R5.7 GFP/Luc HIV-1 reporter cells and the presence of functional virus was assessed by luciferase expression and p24 capture ELISA. (Molnar SM, et al., 2024)
A: Specific transcription factors can be silenced or overexpressed in this cell line using genetic manipulation techniques like siRNA or CRISPR/Cas9. The effect on T-cell leukemia progression can then be assessed by analyzing changes in luciferase activity under control of promoters responsive to these factors, alongside GFP expression to monitor cell health.
A: Gene therapy vectors can be introduced into this cell line to express therapeutic genes or to knockdown disease-related genes. The efficacy of these therapies can be evaluated by monitoring the impact on both GFP and luciferase expression, indicating the success of gene modulation and its effects on cellular functions relevant to T-cell disorders.
A: Yes, by treating these cells with immunomodulatory drugs and monitoring changes in GFP and luciferase expression, researchers can assess the impact of these drugs on T-cell leukemia cell viability and on the expression of genes involved in survival, proliferation, and apoptosis.
A: The dual-reporter system allows for the simultaneous assessment of cell viability (via GFP) and specific gene promoter activity (via luciferase) under various treatment conditions. This enables a comprehensive evaluation of drug effects on T-cell function and gene expression in a single experiment.
A: This cell line can be stimulated with various agents known to activate T-cells, such as anti-CD3 and anti-CD28 antibodies. The activation can then be monitored by measuring luciferase activity, reflecting the transcriptional activity of NF-κB or NFAT, and GFP fluorescence can provide a visual and quantitative measure of the overall cellular response.
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GFP enables non-invasive live tracking of cell viability and proliferation in the GFP/Luc Reporter Cell Line - Jurkat, preserving cell health for longitudinal studies.
GFP/Luc Reporter Cell Line - Jurkat is known for its high transfection efficiency, which is ideal for introducing new genes or siRNA, expanding its use in genetic studies.
The ability to use both GFP and luciferase enables multiplexing with other assays or markers, significantly enhancing the analytical capabilities of the GFP/Luc Reporter Cell Line - Jurkat.
GFP/Luc Reporter Cell Line - Jurkat combines GFP and luciferase, allowing for both qualitative visual monitoring and quantitative bioluminescent measurements. This dual functionality enhances the flexibility and depth of cellular studies.
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