GFP/Luc Reporter Cell Line - SNU-398

SNU-398 is a human hepatocellular carcinoma (HCC) cell line established from a primary liver tumor and is widely utilized in studies of liver cancer biology and therapeutic development. The cell line exhibits characteristics representative of poorly differentiated hepatocellular carcinoma and has been employed extensively to investigate molecular mechanisms involved in hepatocarcinogenesis, tumor progression, and treatment response. SNU-398 cells harbor distinct genetic alterations commonly observed in HCC, making them a valuable experimental model for evaluating oncogenic signaling pathways and targeted therapeutic strategies. The GFP/Luc Reporter Cell Line - SNU-398 is generated through stable introduction of green fluorescent protein (GFP) and firefly luciferase (Luc) reporter genes into the parental SNU-398 cells. This dual-reporter system enables convenient fluorescence-based visualization and highly sensitive bioluminescence detection while maintaining the essential biological and growth characteristics of the original cell line.

The SNU-398-GFP/Luc reporter cell line is widely applied in liver cancer research, particularly for studying tumor growth, invasion, and therapeutic response in both in vitro and in vivo models. Luciferase expression allows non-invasive longitudinal monitoring of tumor burden and disease progression through bioluminescence imaging, providing a sensitive approach for evaluating treatment efficacy in xenograft studies. GFP expression facilitates direct observation of cell proliferation, migration, and localization in cultured cells and tissue specimens. This model is particularly useful for screening anti-cancer compounds, assessing targeted therapies, and investigating mechanisms of drug resistance in hepatocellular carcinoma. In addition, the dual-reporter platform supports studies of oncogenic signaling networks, tumor microenvironment interactions, cancer stem cell-related processes, and metastatic behavior. With stable reporter expression and reliable imaging performance, the SNU-398-GFP/Luc cell line provides a robust and reproducible tool for preclinical drug evaluation and translational research focused on hepatocellular carcinoma.