GFP/Luc Reporter Cell Line - KMH-2

KMH-2 is a human Hodgkin lymphoma cell line established from a patient with classical Hodgkin lymphoma and is widely used as an experimental model for studying the molecular and cellular mechanisms underlying this malignancy. The cell line exhibits phenotypic and genetic characteristics associated with Hodgkin and Reed–Sternberg (HRS) cells, including constitutive activation of signaling pathways involved in cell survival, proliferation, and immune regulation. As a representative model of classical Hodgkin lymphoma, KMH-2 has been extensively utilized in investigations of tumor biology, immune evasion, and therapeutic response. The GFP/Luc Reporter Cell Line - KMH-2 is generated through stable integration of green fluorescent protein (GFP) and firefly luciferase (Luc) reporter genes into the parental KMH-2 cells. This dual-reporter system enables sensitive fluorescence-based visualization and quantitative bioluminescence imaging while maintaining the biological characteristics and growth properties of the original cell line.

The KMH-2-GFP/Luc reporter cell line is broadly applied in hematologic malignancy research and preclinical therapeutic evaluation. Luciferase expression enables non-invasive monitoring of tumor engraftment, expansion, and treatment response in lymphoma xenograft models through longitudinal bioluminescence imaging. GFP expression facilitates visualization of cellular distribution, proliferation, and tumor localization in both in vitro experiments and ex vivo tissue analyses. This model is particularly valuable for assessing the efficacy of targeted therapies, antibody-based treatments, immune-modulating agents, and combination therapeutic strategies for Hodgkin lymphoma. In addition, the dual-reporter platform supports mechanistic studies of tumor–immune interactions, cytokine signaling, immune checkpoint regulation, and pathways contributing to treatment resistance. The stable expression of GFP and luciferase provides a robust and reproducible system for quantitative analysis of lymphoma progression, making the KMH-2-GFP/Luc cell line a reliable tool for translational research and preclinical drug development in Hodgkin lymphoma.