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Capitalizing on years of plant transformation expertise and an advanced technical platform, Creative Biogene has developed a robust and reproducible transformation system tailored to watermelon. Our services are ideal for gene function analysis, resistance gene introduction, and new cultivar development, offering strong technical support for both research and commercial applications.
Watermelon (Citrullus lanatus) belongs to the Cucurbitaceae family and has significant economic value. It is highly favored for its sweet, juicy fruit and wide varietal diversity, which drives increasing breeding demands. However, conventional breeding is constrained by long cycles, low efficiency, and poor trait stability. Genetic transformation presents a promising alternative for trait improvement, resistance gene incorporation, and mechanistic studies, though consistently low transformation efficiency has limited its broader application.
Despite ongoing research efforts, reported transformation efficiencies in watermelon generally remain below 2%, often confined to specific genotypes or laboratory conditions. Factors such as explant type, hormonal balance, bacterial strain, and co-cultivation conditions significantly impact transformation success. A reproducible and broadly applicable transformation platform is thus essential.
Creative Biogene offers a streamlined and customizable Citrullus lanatus transformation workflow, suitable for gene discovery, trait engineering, candidate gene validation, and gene-editing applications. Our end-to-end service includes vector construction, explant preparation, transformation, regeneration, molecular validation, and stable line recovery.
1. Agrobacterium-Mediated Transformation
We utilize an optimized Agrobacterium-mediated approach using cotyledonary node or hypocotyl segment explants. These tissues undergo pre-induction treatments to activate cell division potential and establish a highly responsive shoot organogenesis system, minimizing callus formation and promoting efficient regeneration.
Strains such as Agrobacterium tumefaciens EHA105 or GV3101 are commonly used, carrying binary vectors harboring target genes. Infection and co-cultivation parameters are systematically optimized for maximal T-DNA delivery and expression. Customization of vectors, promoters, and selection markers is supported based on project requirements.
2. Molecular Validation Services
To ensure the authenticity and expression of transgenes, we provide comprehensive molecular verification, including:
Optional downstream analyses such as transcriptomics, DNA methylation profiling, and expression product quantification are also available, supporting full-chain validation from gene to phenotype.
3. Advanced Customization Options
We offer a suite of advanced transformation solutions tailored to your project goals, including:
Suppose you require specialized vector construction, promoter engineering, or transformation enhancement through regeneration regulators such as BBM/WUS or GRF-GIF. In that case, Creative Biogene will provide a tailored, project-specific technical solution to ensure optimal outcomes.
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