Sesbania Cannabina Transformation Service
Creative Biogene offers a full-scope transformation pipeline for Sesbania cannabina, integrating efficient Agrobacterium-mediated transformation with advanced tissue culture and molecular verification systems.
Sesbania cannabina is an annual herbaceous legume native to warm climates, typically growing up to 3–3.5 meters tall. With strong resistance to salinity, waterlogging, and poor soils, it is an ideal pioneer green manure crop for saline-alkaline land improvement. Beyond agriculture, its industrial and pharmaceutical potential has garnered increasing attention. The bast fibers from Sesbania stems exhibit excellent tensile strength and corrosion resistance, outperforming traditional jute fibers. Additionally, Sesbania gum, extracted from seed endosperm, is a galactomannan polysaccharide rich in D-galactose and D-mannose, widely applied as a natural food emulsifier, thickener, and stabilizer. Despite its ecological, agronomic, and industrial importance, research into Sesbania genetic transformation remains scarce. Limited genetic diversity and transformation efficiency have constrained functional genomics and breeding progress.

Our Service Portfolio
Creative Biogene has developed a reliable and customizable Sesbania genetic transformation platform, enabling precise genetic modification and supporting various research objectives in molecular breeding, functional gene discovery, and metabolic engineering.
| Project Type | Technical Highlights |
| Transformation System Development | Tailored Agrobacterium-mediated transformation protocols for Sesbania cannabina, optimized for different explant types and genotypes. Key parameters include bacterial strain selection, co-cultivation conditions, and selection regimes. |
| Regeneration Optimization | Establishment of high-efficiency regeneration systems through precise manipulation of plant growth regulators to support shoot induction and rooting. |
| Stable Transgenic Line Generation | Delivery of healthy, stable Sesbania transformants verified by molecular assays (PCR, qPCR, sequencing) and phenotypic assessments. |
| Gene Function Studies | Support for transgenic overexpression, RNAi-mediated gene silencing, and CRISPR/Cas9 genome editing to accelerate functional genomics research. |
| Trait-Targeted Improvement | Integration of target traits such as stress tolerance, growth enhancement, and metabolite optimization through precise gene manipulation strategies. |
| Custom Project Support | End-to-end project outsourcing, including gene construct design, vector assembly, transformation, and validation. Customization is available for specific research or breeding goals. |
Key Advantages of Sesbania Cannabina Transformation Service
- Legume Transformation Expertise: Extensive experience with other Fabaceae crops enables rapid protocol adaptation for Sesbania transformation.
- Optimized Explant System: Identifies the most responsive tissues (e.g., cotyledon, hypocotyl, stem segments) through pre-culture evaluation to enhance transformation efficiency.
- High-Quality Regeneration Pipeline: Fine-tuned hormone combinations ensure robust regeneration, with healthy shoot formation and high rooting rates.
- Custom Protocol Development: Delivers cultivar-specific transformation strategies, including strain screening, infection optimization, and stress mitigation during co-culture.
- Comprehensive Molecular Validation: Includes PCR confirmation, GUS staining, antibiotic resistance assays, Southern blotting, and gene expression analysis.
Application Scenarios
- Stress Resistance Trait Improvement: Introduces genes related to salt, drought, and flood resistance to enhance Sesbania's adaptability for land restoration and agriculture in marginal areas.
- Bioactive Compound Biosynthesis: Explores and engineers pathways related to valuable compounds such as quercetin, kaempferol, and sesbagrandiforian A/B.
- Functional Genomics and Gene Discovery: Supports in-depth research into gene function related to fiber development, metabolic regulation, or stress signaling.
- Medicinal Value Exploration: Enables production of transgenic lines enriched in galactomannan derivatives and novel secondary metabolites for drug discovery and therapeutic development.
- Industrial Fiber Optimization: Investigates and enhances bast fiber traits such as tensile strength and durability to develop alternatives to traditional industrial fibers.
Contact Us
If you are looking to initiate genetic transformation in Sesbania cannabina, optimize callus induction, or apply gene editing techniques for breeding and functional studies, Creative Biogene is your ideal partner. Our Plant Molecular Biology Platform offers customized services and expert support to accelerate your project from concept to a stable transgenic line with reliable outcomes and scientific rigor.
References:
- Mokhtar FA, Ahmed M, Al Dhanhani AS, et al. Distribution, Phytochemical Insights, and Cytotoxic Potential of the Sesbania Genus: A Comprehensive Review of Sesbania grandiflora, Sesbania sesban, and Sesbania cannabina. Pharmaceuticals (Basel). 2025;18(1):64.
- Luo H, Wang X, You C, et al. Telomere-to-telomere genome of the allotetraploid legume Sesbania cannabina reveals transposon-driven subgenome divergence and mechanisms of alkaline stress tolerance. Sci China Life Sci. 2024;67(1):149-160.
* For research use only. Not intended for any clinical use.