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Celery (Apium graveolens), belonging to the Apiaceae family, is a globally important vegetable utilized for its leaves, stems, and roots, with widespread applications in culinary, flavoring, and medicinal fields. As a structurally complex dicotyledonous plant, celery has a diploid chromosome number of 2n=22, a relatively large genome (approximately 3.18 Gb), and a high proportion of repetitive sequences, presenting significant challenges for genetic improvement.
Currently, the major production challenges facing celery include frequent disease and pest infestations (such as Fusarium wilt and early blight), poor stress tolerance (particularly sensitivity to saline-alkaline conditions and high temperatures), lengthy breeding cycles, and complex genetic backgrounds. Although traditional hybridization breeding has improved certain traits to some extent, it still exhibits limitations such as low efficiency, minimal selection pressure, and unstable trait inheritance. There is an urgent need to leverage molecular breeding and genetic engineering technologies to achieve breakthroughs, especially in improving complex traits like resistance, nutritional quality, and volatile compound accumulation.
Genetic transformation, as an essential tool in modern breeding, has been widely applied to crops such as rice, wheat, soybean, and tomato, while research on celery genetic transformation began relatively later. In recent years, with the gradual completion of celery genome mapping and ongoing functional gene discovery, increasing numbers of researchers have focused on key regulatory genes related to disease resistance, stress tolerance, and nutritional quality in celery, such as DREB, HSF, MaT, VIP, and PAL, establishing a solid foundation for celery genetic transformation and functional research. Therefore, developing an efficient, stable, and scalable celery genetic transformation system has become a critical pathway for advancing functional gene analysis, molecular design breeding, and specialized resource development for this species.
Based on this need, Creative Biogene has leveraged years of transformation technology experience and systems biology analytical capabilities to launch comprehensive celery genetic transformation services for research and breeding applications.
1. Transformation System Development
For different celery varieties, we have developed proprietary transformation systems. By screening various explants (such as leaves, stem segments, hypocotyls) and optimizing culture media formulations, combined with temperature and light conditions, we have established efficient genetic transformation platforms.
2. Gene Overexpression and Silencing
We provide overexpression and RNA interference (RNAi) services required for gene function research. By constructing specific expression vectors to introduce target genes or their antisense sequences, we enable functional validation of key genes in celery.
3. Gene Editing
Using Gene Editing technology, we can precisely edit specific genes in the celery genome. This includes knocking out unfavorable trait genes and repairing beneficial gene mutations.
4. Genetic Improvement and New Variety Development
Integrating modern molecular breeding techniques, we are dedicated to celery genetic improvement work. This encompasses enhanced disease resistance (such as Fusarium wilt resistance), increased yield, and improved quality.
With ongoing advancements in biotechnology, celery genetic improvement is entering a new era of opportunity. Creative Biogene is committed to providing efficient and reliable celery genetic transformation services to research institutions and breeding enterprises, supporting the sustainable development of the celery industry. We look forward to collaborating with you to drive innovation and breakthroughs in celery breeding.
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