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Lotus japonicus Transformation Service

To accelerate the functional genome research and application of crops, Creative Biogene develops various model plant transformation system, such as leguminous, gramineous, cruciferous plants. Especially considering the high challenge and low frequency of leguminous plants transformation and regeneration, we offer several Lotus japonicas varieties transformation and genome editing services, including but not limited to Gifu B-129, Miyakojima MG-20, Funakura B581.

Legumes, which include many of the important crop plants utilized as food for human consumption, forages, herbal medicines and oil materials, have significant and unique characteristics, such as the formation of compound leaves and zygomorphic flowers, accumulation of seed storage proteins, production of legume-specific flavonoids and symbiotic nitrogen fixation.

A leguminous model plant is expected to provide the framework within which the molecular mechanisms that underlie the legume-specific character can be clarified and leguminous crops in general can be improved. Lotus japonicus is a model plant for genome studies in legumes, particularly in reference to rhizobial and arbuscular mycorrhizal symbiosis. Small genome size of about 470 Mb, diploid genome with 6 haploid chromosomes, a short life cycle of about 2 to 3 months and its perennial nature makes it a convenient plant to study.

Agrobacterium-mediated Lotus japonicas transformation service Figure 1. Agrobacterium-mediated Lotus japonicas transformation service

Custom service offered by Creative Biogene

  • Free consultation
  • Experimental design
  • Vector Design/Construction (overexpression, knockdown, knockout)
  • Transgene validation
  • Transgene copy number estimation
  • Single/multiple gene knockout
  • Virus induced gene silencing

Creative Biogene provides plant transformation and genome editing service of various species, to accommodating the diverse needs of our clients from all over the world. Customer-tailored service about molecular and tissue culture experiments are also available. Besides, our staff has extensive experience in the development and optimization of transformation protocols for species where transformation methods are either not available or are highly inefficient.

References:

  1. Sasaki, T. (2013). Stable transformation in lotus japonicus. BIO-PROTOCOL, 3(12).
  2. Aoki, T. (2002). Efficient agrobacterium-mediated transformation of lotus japonicus with reliable antibiotic selection. Plant Cell Reports, 21(3), 238-243.
For research use only. Not intended for any clinical use.

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