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Sorghum Transformation Service

Creative Biogene offers a variety of services for global customers including molecular construction, plant transformation, phenotyping/sampling of GM events, and insert quality analysis. Regular sorghum transformation service of Creative Biogene will offer Tx430/P898012 genotype using standard immature embryo and Agrobacterium-mediated transformation techniques. Protocols to transform additional inbred lines are in development, and can be prioritized based on customer interest.

Sorghum (Sorghum bicolor L.), the fifth most important cereal after wheat, rice, maize and barley, plays a unique role in food security and renewable energy. It is widely used for food especially in Africa and India, and for animal consumption (grain and stover) and as a biofuel energy source in Americas and Australia. In addition, sorghum has been identified as a prospective bioenergy crop and model for C4 grasses. Genetic engineering has the potential to have a great influence on improving sorghum yield and digestibility. Although sorghum genetic transformation has been studied for many decades, a highly efficient and comprehensive system has remained elusive till date due to accumulation of phenolic compounds, lack of model genotypes, low regeneration frequency and loss of regeneration potential through sub-cultures. It’s necessary to develop an efficient and broadly applicable protocol for sorghum transformation.

Agrobacterium-mediated transformation using immature embryo and differentiating embryogenic callus is the most common method for sorghum (Figure 1).

Sorghum Transformation ServiceFigure 1. Agrobacterium-mediated sorghum cv. Tx430 using immature embryo.

Benefits and feature

  • Fast turnaround time and cost effective
  • One stop service from project design, molecular construction, transformation to characterization
  • Complete service including overexpression, gene silencing, genome editing.

With years of experience in molecular biology and plant transformation, Creative Biogene offers sorghum transformation service for Tx430/p898012 genotype, as well as protocol development service for client’s inbred line or cultivar. We encourage customer to contact us with any suggestions/requests for future services you may have in mind or potential research collaborations you may be considering that our extensive expertise in plant transformation could help with. Please feel free to contact us at . We are waiting for your interest projects.

Reference:

  1. Liu, G. (2015). A robust tissue culture system for sorghum [sorghum bicolor (l.) moench]. South African Journal of Botany, 98, 157-160.
For research use only. Not intended for any clinical use.

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