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Soybean (Glycine max (Linn.) Merr.) is one of the world's most important oilseed and protein crops, playing a critical role in human nutrition, animal feed, industrial applications, and biofuel production. To meet the increasing demand for high-yield, stress-tolerant, and nutritionally improved varieties, genetic engineering and genome editing technologies provide powerful tools for crop improvement.
Creative Biogene has developed highly efficient soybean transformation and genome editing platforms, supporting all aspects from molecular construction to plant regeneration and growth. Our services cover major soybean genotypes, including Williams 82, Thorne, Jack, Tianlong 1, Bert, and more. Whether you aim to perform traditional transgenesis or CRISPR-based gene editing, we provide reliable, scalable, and cost-effective solutions tailored to your research needs.
Figure 1. Agrobacterium-mediated soybean (Williams 82) transformation
Traditional breeding methods have made significant contributions to soybean improvement, but face limitations such as:
By contrast, plant genetic transformation enables precise control over target traits, accelerates breeding cycles, and allows the generation of novel and genetically diverse genotypes. Researchers use soybean transformation for:
Creative Biogene primarily uses the Agrobacterium-mediated cotyledonary node method, which offers high reproducibility and efficiency. Additionally, we provide CRISPR/Cas9 gene editing services to generate transgene-free, commercially viable soybean lines, as well as a hairy root transient expression system for functional studies or plant-based protein production.
Key Steps of Soybean Transformation:
| Step | Description |
| Seed sterilization & imbibition | Prepare explants for transformation |
| Agrobacterium preparation | Strains: EHA101, EHA105, GV3101, AGL1, C58C1 |
| Explant infection | Half-seed cotyledonary explants inoculated |
| Co-cultivation | Agrobacterium interacts with explant cells |
| Initial regeneration | Shoot induction and elongation |
| Plantlet formation & rooting | Formation of healthy transgenic seedlings |
| Screening & validation | Molecular verification, resistance selection |
A workflow diagram is available upon request to illustrate the transformation process.
Creative Biogene differentiates itself through end-to-end project support, technical expertise, and high efficiency:
| Package | Content | Deliverables | Timeline |
| Standard Transformation | Traditional T-DNA insertion | T0 positive plants ≥10 independent lines | 6–8 months |
| CRISPR Full Package | Vector construction + transformation | T0 positive lines ≥3 per target, sequencing & mutation info | 4–6 months |
| Transient Expression | Hairy root system | Gene function validation or protein expression | 7–15 days |
Additional customization is available according to variety, target gene, and experimental goals.
Creative Biogene is committed to supporting your soybean research with high-quality, cost-effective, and reliable transformation and genome editing services. Contact us today to discuss your project and receive a free consultation with our plant transformation experts.
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