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Barley Transformation Service

Creative Biogene can help to advance research in many areas of plant science by providing functional characterization of genes of interest and by providing knock-out mutants using CRISPR/Cas9 based technologies. We offer a complete barley transformation service from experiment design, construct assembly, Agrobacterium-mediated transformation to transformants screening.

The genetic transformation of cereal crops has been widely studied as a tool for crop improvement and as a vital part of the development of functional genomics resources. Barley ranks fourth among the cereals in quantity produced and is grown in around 100 countries worldwide. As well as being an important crop in its own right, barley has also been suggested as a valuable diploid model for the more complex, hexaploid cereal crop, wheat. Also, the demand for efficient crop transformation systems continues to grow, with new genome editing technologies adding to that demand. Thus, it's necessary to develop high-throughput barley transformation systems to facilitate the exploitation and application of functional genomics resources in barley and wheat.

Workflow of barley transformation

  • Preparation of plant material, culture media.
  • Collection and sterilization of immature seed.
  • Isolation and preculture of immature embryos
  • Agrobacterium inoculation (AGL1) and immature embryo co-cultivation
  • Callus induction
  • Regeneration and rooting

Agrobacterium-mediated barley transformation Figure 1. Agrobacterium-mediated barley transformation

Creative Biogene assists researchers by providing barley transformation capacity ranging from use of a single construct to regenerate 5-10 independent lines to much larger scale experiments for commercial and academic organization. We have molecular and bioinformatics expertise within the group, to also undertake vector and construct design and analysis of the transgenic plants as required. For further information, please feel free to contact us at .

Reference:

  1. Alison, Hinchliffe. (2019). Agrobacterium-mediated transformation of barley immature embryos. Methods in Molecular Biology.
For research use only. Not intended for any clinical use.

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