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Creative Biogene provides comprehensive barley transformation services designed to support functional genomics, trait engineering, and precision gene editing. Leveraging CRISPR/Cas9 and other advanced genome-editing technologies, we enable the generation of knock-out, knock-in, and overexpression lines in barley—serving both basic research and applied breeding goals. Our service encompasses the full workflow, from experimental design and vector construction to Agrobacterium-mediated transformation, transgenic plant regeneration, and molecular validation.
Barley (Hordeum vulgare) ranks fourth among global cereal crops in terms of production and is cultivated across more than 100 countries. In addition to its agronomic importance, barley serves as an ideal diploid model for more complex cereals like wheat due to its relatively simpler genome. The advancement of cereal transformation systems has played a vital role in crop improvement and functional genomics studies. As genome editing technologies such as CRISPR/Cas9, base editing (ABE/CBE), and prime editing (PE) evolve, there is a growing demand for high-efficiency, high-throughput transformation platforms.
At Creative Biogene, we address this demand by offering a robust barley transformation platform capable of handling diverse vector types and research objectives, including gene overexpression, RNA interference (RNAi), and precise genome editing.
| Transformation Type | Vector Type | Deliverables | Turnaround Time | Remarks |
| Standard Transformation | Overexpression / RNAi / amiRNA vectors | ≥10 independent T0 positive lines | ~7 days | 1–2 targets require ~7 days; timeline may extend with additional targets |
| CRISPR-based Transformation | CRISPR/Cas9 vectors | 3–5 independent T0 positive lines | ~15 days | For barley, it includes sequencing and mutation identification (~3 weeks detection) |
| Precise Gene Editing | ABE / CBE / PE vectors | 3–5 independent T0 positive lines with edits | 4–5 months | Includes verification of edits and mutation site analysis |
| Custom Gene Editing | Non-ABE/CBE/PE vectors | 3–5 independent T0 positive lines with edits | 3–4 months | Timeline varies by genotype; challenging cultivars require separate evaluation |
| Genome Editing Transformation | General genome editing vectors | 3–5 independent T0 positive lines | 4–5 months | Includes genotyping and molecular analysis |
Figure 1. Agrobacterium-mediated barley transformation process.
Creative Biogene supports both small-scale and large-scale barley transformation projects, from regenerating 5–10 independent transgenic lines to hundreds, depending on your research needs. Our service is highly customizable and can be adapted for both academic studies and commercial applications. In addition to transformation, we offer in-house molecular biology, construct design, and bioinformatics capabilities to support vector assembly, genotyping, and data interpretation of transgenic lines.
Whether your objective is gene function characterization, trait discovery, or genome editing validation, we offer a one-stop solution with reliable outcomes and expert consultation throughout the project.
To learn more about our barley transformation capabilities or to discuss your specific project needs, please contact us. Our technical team will be happy to assist you with tailored solutions.
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