Grape Transformation Service
Grapes are one of the world's most widely cultivated and economically valuable fruit crops. Creative Biogene is dedicated to building an efficient, stable grape genetic transformation platform, particularly addressing the low transformation efficiency challenges of cultivated grape varieties by developing highly adaptable transformation systems. We support multi-level application needs, including gene function validation, metabolic engineering modification, and resistance trait development, helping researchers advance grape genetic improvement and precision breeding.
Grape (Vitis vinifera) not only dominates the fruit consumption and wine production sectors but has also become a focal crop for functional food research due to its rich polyphenolic compounds. According to 2022 data from the International Organisation of Vine and Wine (OIV), global grape cultivation area reaches 7.3 million hectares, with production exceeding 80 million tons and an annual value of approximately 60 billion dollars. Grape-derived products not only possess significant economic value, but their bioactive substances, such as proanthocyanidins and resveratrol, also exhibit numerous biological activities, including antioxidant, anti-inflammatory, antimicrobial, and anticancer properties, making them hotspots for food nutritional functionality development. With intensifying global climate change and increasing consumer health awareness, the demand for genetic improvement of grape germplasm resources and functional gene research has become increasingly urgent, with genetic transformation technology becoming a key breakthrough for breeding bottlenecks.
Service Content Overview
Creative Biogene's grape genetic transformation services address various breeding and research needs, providing the following core content:
- Grape Genetic Transformation System Development and Optimization: Establishing efficient, stable transformation processes for wine varieties (such as Cabernet Sauvignon, Chardonnay), table varieties (such as Kyoho, Summer Black), and difficult-to-transform local specialty varieties, including explant screening, infection system optimization, and regeneration system reconstruction.
- Exogenous Gene Overexpression and RNA Interference: Providing stable transgenic grape construction, including target gene expression enhancement, inhibition mechanism studies, metabolic pathway modification, etc., to support functional gene analysis and metabolic engineering optimization.
- Gene Editing and Site-directed Mutation: Offering gene editing services via both transgenic and non-transgenic pathways, covering direct delivery of RNP to protoplasts, targeted knockout/knock-in, mutation verification, precise improvement of disease resistance, stress tolerance traits, and more.
- Genetic Improvement and Functional Validation Services: Combining physiological and biochemical analysis, molecular verification, and phenotype assessment to achieve multi-dimensional improvement goals, including disease resistance, stress tolerance, and fruit quality.
Technical Highlights
- Customized Systems for Difficult-to-transform Cultivated Varieties: Employing multi-factor optimization strategies to improve variety regeneration and transformation rates, successfully establishing transformation models suitable for different genetic backgrounds.
- CRISPR Editing System Supporting Multiple Pathways: Achieving parallel selection of T-DNA-mediated and direct RNP delivery pathways, balancing efficiency and commercial feasibility.
- High-activity Expression Vectors and Reporting Systems: Including pCAMBIA, pBI121 series expression frameworks, and supporting reporter genes such as GUS, GFP, and LUC for precise visual verification of gene function.
- Adaptable Targeted Improvement for Multiple Trait Types: Addressing key breeding goals such as powdery mildew resistance, drought tolerance, fruit aroma enhancement, and anthocyanin content increase.
Service Process
- Customer requirement investigation and project feasibility analysis
- Transformation vector construction and verification
- Infection transformation experiments and screening system establishment
- Regenerated plant acquisition and positive verification
- Target trait detection and data analysis
- Report delivery and follow-up technical support
Service Clients
- Fruit tree breeding research institutions and university laboratories
- Food and nutrition research enterprises
- Grape functional gene research groups
- Disease and stress-resistant variety development units
- Precision agriculture and molecular breeding platforms
Case Study
Contact Us
If you are conducting grape genetic improvement research or expect to achieve precise molecular-level regulation in specialty varieties, Creative Biogene will be your trustworthy partner. We possess first-class experimental platforms, an experienced team of molecular breeding experts, and a rigorous and efficient project management system to help you effectively achieve grape gene function research and industrialized breeding goals.
References:
- Milinčić DD, Stanisavljević NS, et al. The Bioaccessibility of Grape-Derived Phenolic Compounds: An Overview. Foods. 2025;14(4):607.
- Cantu D, Massonnet M, Cochetel N. The wild side of grape genomics. Trends Genet. 2024;40(7):601-612.
- Zhou J, Li D, Wang G, et al. Application and future perspective of CRISPR/Cas9 genome editing in fruit crops. J Integr Plant Biol. 2020;62(3):269-286.
* For research use only. Not intended for any clinical use.
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