Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells.
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Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells. They can up-regulate the endogenous miRNA activity by utilizing the natural miRNA machinery. Creative Biogene’s microUP™ miRNA agomir is designed to mimic mature miRNAs, and chemically modified to increase their stability and activity. They are recommended for miRNA functional in vitro and in vivo studies.
Figure 1. Mechanism of microUP™ miRNA agomir.
The antisense strand of the agomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. It exhibits enhanced cellular uptake, stability and regulatory activity in vivo. They can up-regulate the corresponding endogenous miRNAs by either local or systemic injection into the animals.
Figure 2. Cells are incubated with equal amount of microUP™ miRNA agomir and miRNA mimic. qRT-PCR is performed after 2 days.
Figure 3. qRT-PCR and western blot assays show the mRNA (A) and protein (B) expressions of TOB1 after transfection with agomir-NC and agomir-25.
Our microUP™ miRNA agomir has unparalleled advantages
Resuspension Protocol
In Vivo Delivery
Recommended amounts of microUP™ miRNA agomir for your in vivo study.
References | miRNA | Animal | Delivery | Doses | Volume | Injection Times | Detection |
Min-Jie Ni, et al. PLoS ONE. 2011 | mil-HongrES2 | 7~10 days SD rat | cauda epididymis injection | 4 nmol per side | N/A | N/A | 3 days after injection |
Dong Li, et al. JBC. 2011 | miR-99a | nude mice | tumor mass injection | 10 nmol | 0.1 ml per injection | every three days for two weeks | 2~4 weeks after injection |
Jin Hou, et al. Cancer Cell. 2007 | miR-199a/b-3p | nude mice | tumor mass injection | 10 nmol | 0.1 ml per injection | once every three days for two weeks | 2~4 weeks after injection |