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Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells. They can up-regulate the endogenous miRNA activity by utilizing the natural miRNA machinery. Creative Biogene’s microUP™ miRNA agomir is designed to mimic mature miRNAs, and chemically modified to increase their stability and activity. They are recommended for miRNA functional in vitro and in vivo studies.
Figure 1. Mechanism of microUP™ miRNA agomir.
The antisense strand of the agomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. It exhibits enhanced cellular uptake, stability and regulatory activity in vivo. They can up-regulate the corresponding endogenous miRNAs by either local or systemic injection into the animals.
Our microUP™ miRNA agomir has unparalleled advantages
Resuspension Protocol
In Vivo Delivery
Recommended amounts of microUP™ miRNA agomir for your in vivo study.
| miRNA | Animal | Delivery | Doses | Volume | Injection Times | Detection | References |
| mil-HongrES2 | 7~10 days SD rat | cauda epididymis injection | 4 nmol per side | N/A | N/A | 3 days after injection | Min-Jie Ni, et al. PLoS ONE. 2011 |
| miR-99a | nude mice | tumor mass injection | 10 nmol | 0.1 ml per injection | every three days for two weeks | 2~4 weeks after injection | Dong Li, et al. JBC. 2011 |
| miR-199a/b-3p | nude mice | tumor mass injection | 10 nmol | 0.1 ml per injection | once every three days for two weeks | 2~4 weeks after injection | Jin Hou, et al. Cancer Cell. 2007 |
Case Study 1
Figure 2. Cells are incubated with equal amount of microUP™ miRNA agomir and miRNA mimic. qRT-PCR is performed after 2 days.
Case Study 2
Figure 3. qRT-PCR and western blot assays show the mRNA (A) and protein (B) expressions of TOB1 after transfection with agomir-NC and agomir-25.
Case Study 3
In order to study whether miR-130b-3p can affect gastric cancer (GC) tumor formation and angiogenesis in vivo, the researchers injected NUGC-3 and HGC27 cells into nude mice to conduct subcutaneous tumor formation experiments. The study results showed that the expression of miR-130b-3p increased in tumor-bearing nude mice injected with miR-130b-3p agomir, while treatment with miR-130b-3p antagomir reduced the expression of miR-130b-3p in NUGC-3 and HGC27 cells (Figure 4a). Tumor volume and weight increased significantly (Figure 4b,c), Bcl-2 and VEGF expression increased, Bax and cleaved caspase3 expression decreased (Figure 4d,e), and CD31 expression increased (Figure 4f) upon injection of miR-130b -3p agomir in nude mice. Opposite effects on these markers were observed in nude mice injected with miR-130b-3p agomir. These results indicate that miR-130b-3p promotes GC tumor formation and angiogenesis in vivo.
Figure 4. miR-130b-3p induces GC tumor formation and angiogenesis in vivo. (Zhang Y, et al., 2020)
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