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microUP™ miRNA Agomir


Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells.

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microUP™ miRNA Agomir

Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells. They can up-regulate the endogenous miRNA activity by utilizing the natural miRNA machinery. Creative Biogene’s microUP™ miRNA agomir is designed to mimic mature miRNAs, and chemically modified to increase their stability and activity. They are recommended for miRNA functional in vitro and in vivo studies.

Figure 1. Mechanism of microUP™ miRNA agomir.

The antisense strand of the agomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. It exhibits enhanced cellular uptake, stability and regulatory activity in vivo. They can up-regulate the corresponding endogenous miRNAs by either local or systemic injection into the animals.

Figure 2. Cells are incubated with equal amount of microUP™ miRNA agomir and miRNA mimic. qRT-PCR is performed after 2 days.

Figure 3. qRT-PCR and western blot assays show the mRNA (A) and protein (B) expressions of TOB1 after transfection with agomir-NC and agomir-25.

Our microUP™ miRNA agomir has unparalleled advantages

  1. Cover all human, mouse, rat miRNAs
  2. Optimized modifications for stable utility and easy transfection
  3. Long-term (up to 6 weeks) up-regulation of the corresponding endogenous miRNAs
  4. No need of virus or vector for delivering
  5. Ready for in vivo and in vitro studies
  6. Various scales and grades for your application
  7. microUP™ miRNA agomir negative control is available

Resuspension Protocol

  1. Briefly centrifuge tubes containing miRNA agomir to ensure that the miRNA pellet is located at the bottom of the tube.
  2. Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: dissolve 1 OD miRNA agomir to 20 µM using 125 µl DEPC H2O (or RNase-free water).
  3. Pipette the solution up and down 3-5 times (or vortex briefly).
  4. Briefly centrifuge tubes containing miRNA agomir to ensure that the solution is collected at the bottom of the tube.
  5. Aliquot the miRNA agomir into small volumes and store at ≤ -20°C. miRNA agomir is stable (for 6 months under the specified storage condition). For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times.

In Vivo Delivery

Recommended amounts of microUP™ miRNA agomir for your in vivo study.

ReferencesmiRNAAnimalDeliveryDosesVolumeInjection TimesDetection
Min-Jie Ni, et al. PLoS ONE. 2011mil-HongrES27~10 days SD ratcauda epididymis injection4 nmol per sideN/AN/A3 days after injection
Dong Li, et al. JBC. 2011miR-99anude micetumor mass injection10 nmol0.1 ml per injectionevery three days for two weeks2~4 weeks after injection
Jin Hou, et al. Cancer Cell. 2007miR-199a/b-3pnude micetumor mass injection10 nmol0.1 ml per injectiononce every three days for two weeks2~4 weeks after injection

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