Poplar Transformation Service
Creative Biogene provides professional transgenic poplar services, focusing on the use of advanced genetic engineering technologies to cultivate poplar varieties with superior traits such as insect resistance and stress tolerance. Utilizing globally advanced transformation methods, including Agrobacterium-mediated transformation, we help researchers and breeders achieve breakthroughs in poplar species' genetic improvement and application research. Our goal is to enhance poplar trees' adaptability, resistance, and productivity across diverse environments.
Poplar (Populus spp.) is one of the most widely cultivated and fast-growing woody species in temperate regions, renowned for its straight trunk, high-quality timber, and resilience to adverse environmental conditions. It is extensively used in timber production, ecological restoration, landscaping, and other sectors. Due to the diversity of poplar species and regional differences in climate, selecting suitable cultivars is crucial. In recent years, pest outbreaks have significantly impacted poplar plantations, making genetic engineering a promising tool for improving insect resistance. While model poplar species such as Populus alba × glandulosa and Populus tomentosa have established transformation systems, efficient genetic transformation protocols are still lacking for region-specific elite varieties.

Service Details
Creative Biogene establishes and optimizes the genetic transformation system for poplar, offering precise and efficient transgenic services to research institutions and breeding enterprises worldwide.
1. Establishment of Genetic Transformation System
Creative Biogene provides a complete solution for building a robust genetic transformation system for poplar. Our service encompasses the selection of suitable explants, optimization of culture media, and the design of transformation conditions. Key optimizations include:
- Explant selection (such as stem segments, leaf discs, etc.)
- Agrobacterium suspension concentration, infection duration, and co-cultivation parameters
- Improvement of transformation efficiency through systematic optimization tests, ensuring both high effectiveness and stability of gene integration
2. Development of Transgenic Poplar Lines
We perform targeted transformations using specific insect-resistant genes (e.g., BtCry3Aa) to enhance the pest resistance of poplar varieties. On the basis of successful gene insertion, clients can obtain newly developed poplar lines with multiple advantageous traits, including insect resistance, stress tolerance, and salt-alkali resistance.
3. Molecular Marker Identification and Genotypic Analysis
We offer comprehensive molecular identification services to verify the presence of transgenes. Using PCR and related techniques, we accurately detect positive transgenic plants. Genotypic analysis is also conducted to ensure the stability and genetic consistency of each transformation event.
Service Highlights of Our Poplar Transformation Service
- High-Efficiency Transformation Technology: Our Agrobacterium-mediated transformation method offers greater stability and efficiency than particle bombardment or electroporation, enabling reliable gene expression across multiple generations.
- Optimized Protocols for Various Poplar Varieties: By analyzing experimental data from multiple poplar species, we provide highly tailored transformation protocols, including optimal bacterial density, infection time, and co-culture conditions, to achieve high transformation efficiency.
- Comprehensive Genotype Verification: Our service includes sensitive PCR-based molecular marker analysis and genotype verification to help clients accurately identify positive transformants and gather reliable phenotypic and planting data.
- Multi-Trait Development Capabilities: Creative Biogene's transgenic technology supports the development of poplar varieties with multiple beneficial traits, such as insect resistance, stress tolerance, fast growth, and salt-alkali resistance, to meet diverse application needs.
Application Fields
- Accelerated Seedling Production: Through our transformation service, breeders can quickly produce poplar seedlings with enhanced traits (e.g., resistance to pests, diseases, or environmental stress), significantly improving seedling production efficiency, especially for high-demand or newly improved cultivars.
- Variety Improvement: Our transgenic technology offers an efficient solution for poplar variety enhancement, enabling breeders to obtain improved varieties with superior traits (e.g., pest resistance, salt tolerance) in a shorter time, thereby increasing market competitiveness.
- Rapid Commercialization: By enabling mass propagation of transgenic poplar, breeders can quickly introduce new varieties to the market and scale up production. Our rapid propagation techniques greatly shorten the time from lab to commercial plantation.
- Agroforestry Integration: Transgenic poplars with enhanced traits such as pest resistance and abiotic stress tolerance are well-suited for integrated agroforestry systems. They increase ecological and economic benefits in applications such as large-scale afforestation, windbreaks, green belts, and timber production.
Case Study

Contact Us
Creative Biogene's Poplar Transformation Services offer advanced and tailored genetic solutions for researchers and industry professionals. Whether the goal is to enhance resistance traits, improve ecological performance, or accelerate commercialization, our reliable and high-efficiency transformation technologies provide strong support for sustainable development in the poplar industry. For inquiries or further details, please contact us—Creative Biogene is your most trusted partner in biotechnology innovation.
References:
- Bannoud F, Bellini C. Adventitious Rooting in Populus Species: Update and Perspectives. Front Plant Sci. 2021;12:668837. Published 2021 May 20.
- Rosso L, Cantamessa S, Bergante S, et al. Responses to Drought Stress in Poplar: What Do We Know and What Can We Learn?. Life (Basel). 2023;13(2):533.
* For research use only. Not intended for any clinical use.