Brassica rapa Transformation Service

In the context of accelerating plant functional gene research and molecular breeding, Creative Biogene has established an efficient genetic transformation system for Chinese cabbage and its related species, based on years of accumulated plant transformation technology and comprehensive tissue culture systems. We provide not only standard Agrobacterium-mediated genetic transformation services but have also accumulated rich experience in developing transformation systems for recalcitrant varieties, gene overexpression and silencing, genome editing, and targeted genetic improvement.

Chinese cabbage

Chinese cabbage (Brassica rapa L. ssp. pekinensis), as an important cruciferous vegetable crop, occupies a significant position in global food security and the vegetable industry. Domesticated in China around 6,000 years ago from non-heading ancestors, it later spread across Asia and evolved into two main heading types with superior traits like cold tolerance, storability, and yield. Due to its genomic characteristics and wide range of related plants, it has become a research model for plant gene function studies, stress biology, metabolic engineering, and other areas. Chinese cabbage has unique research value in polyploid evolution, transcriptional regulation, flowering signaling pathways, and secondary metabolism research. The establishment of genetic transformation systems provides important support for analyzing gene functions, creating new germplasm, and accelerating molecular breeding processes. However, due to factors such as multiple inbred lines, poor tissue culture response, and strong genotype dependence, the genetic transformation efficiency of Chinese cabbage is generally low.

Service Content

Creative Biogene provides comprehensive platform services for Chinese cabbage genetic manipulation, from standard genetic transformation to complex transformation system development, molecular-level functional operations, and targeted improvements, meeting the needs of different research and breeding objectives:

Chinese Cabbage Genetic Transformation (Standard Process)

Primarily using Agrobacterium tumefaciens-mediated methods, while also mastering gene gun and magnetofection techniques that can be adapted to efficient regeneration systems for different varieties
Supporting foreign gene introduction, marker gene co-expression, and selection marker (Bar/NPTII) detection
Providing stable transgenic plants and progeny metabolic and phenotypic analysis services

Transformation System Development (For Special Cultivars)

Customized tissue culture condition optimization for different genetic backgrounds or cultivars (such as highly resistant inbred lines, commercial breeding materials)
Including explant responsiveness screening, induction/differentiation/rooting medium adjustment, resistance agent concentration curve analysis, etc.
Delivering high-efficiency regeneration systems and transformation condition reports to support customer platform construction or independent experimentation

Overexpression and Gene Silencing

Building overexpression systems based on 35S or tissue-specific promoters to achieve enhanced expression of functional genes
Providing RNA interference (RNAi), artificial miRNA, antisense, and other silencing strategies to verify target gene loss-of-function phenotypes
Can be combined with qRT-PCR, protein detection, and metabolite analysis for expression level assessment

Chinese Cabbage Genetic Improvement and Functional Validation

Promoting functional improvement of germplasm resources based on functional gene transformation, combined with field data and metabolic pathway information
Including disease resistance, stress resistance (salt tolerance, cold tolerance, drought), and enhancement of breeding target traits (such as bolting characteristics, nutrient accumulation)
Delivering application-oriented data and stable materials that can be used for commercial development

Technical Advantages and Service Features

Efficient Transformation System: Significantly improved transformation frequency by adapting systems and screening transformation conditions for different genotypes of Chinese cabbage materials, optimizing key parameters such as co-cultivation time, bacterial suspension concentration, and medium composition.
Specialized Differentiation Platform: Established a rapid induction system centered on plant growth regulators (such as 6-BA, IAA, KT), compatible with multiple genotype backgrounds, improving regeneration consistency and stability.
Rigorous Detection System: Employing multi-level molecular detection methods to ensure target gene integration and expression, supporting downstream functional research or breeding applications.
Experienced Technical Team: Possessing rich experience in cruciferous crop transformation and tissue culture, having successfully achieved efficient expression and stable inheritance of multiple genes in Chinese cabbage.

Flexible Service Models

Service Type	Suitable For	Characteristics
Standard Transformation Service	Basic research projects, preliminary validation projects	Mature process, quick turnaround, controllable cost
Customized Transformation System	New variety/difficult material development	Personalized condition development, with optimization report
Molecular Operation Combination Package	Functional research/precision breeding direction	Integrated design of overexpression + RNAi/CRISPR
Genetic Improvement Service	Applied research, commercial potential projects	Providing progeny analysis and field feasibility recommendations

Application Scenarios and Service Outputs

Application Directions:

Gene function validation and expression analysis (including genome editing, RNAi silencing, etc.)
Phenotypic function research and signal pathway analysis
Crop disease resistance and stress tolerance trait improvement
Synthetic biology and metabolic engineering research career

Service Deliverables:

Expression vector construction report and plasmid samples
Transformation-positive plants and regeneration system parameter records
Molecular verification data (PCR profiles, sequencing results, etc.)
Optional subsequent T1 generation seed propagation and analysis services

Case Study

Contact Us

Whether your project involves developing elite Chinese cabbage cultivars, dissecting gene functions, or performing metabolic engineering, our team at Creative Biogene is here to support your goals with reliable transformation systems and professional molecular biology services. Reach out to our Plant Molecular Biology Platform to explore custom strategies for Brassica rapa transformation and accelerate your research with high efficiency and technical excellence.

References:

Dai Y, Sun X, Wang C, et al. Gene co-expression network analysis reveals key pathways and hub genes in Chinese cabbage (Brassica rapa L.) during vernalization. BMC Genomics. 2021;22(1):236.
Okamoto, T. et al. (2021). Chinese Cabbage (Brassica rapa L. var. pekinensis) Breeding: Application of Molecular Technology. In: Al-Khayri, J.M., Jain, S.M., Johnson, D.V. (eds) Advances in Plant Breeding Strategies: Vegetable Crops. Springer, Cham.

* For research use only. Not intended for any clinical use.

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