Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Tomato (Solanum lycopersicum L.) is one of the world's most widely cultivated vegetable crops and a cornerstone of fresh consumption and food processing industries. Due to its short life cycle, relatively small genome, self-pollination ability, and genetic resources, tomato has become not only an economically important crop but also a powerful model system for molecular, biochemical, and physiological research.
Creative Biogene provides a state-of-the-art tomato transformation platform, enabling global researchers to conduct functional genomics studies, validate genome editing tools, and accelerate breeding of elite cultivars with improved traits such as drought tolerance, disease resistance, nutritional quality, and yield performance.
Tomato, with its short growth cycle of 45–100 days, ease of propagation, and well-annotated genome resources, has become an ideal model for both basic plant biology and applied breeding research. Since the first transgenic tomato was generated nearly three decades ago, transformation technologies have been continuously optimized, making tomato an efficient and reliable system for genetic studies.
Today, tomato transformation underpins a wide range of applications, including functional validation of plant genes, CRISPR/Cas-based genome editing and gene replacement, metabolic pathway engineering to enhance nutritional content, and breeding programs aimed at improving resistance to both abiotic and biotic stresses.
Figure 1. Agrobacterium-mediated tomato transformation
Creative Biogene has established an optimized Agrobacterium-mediated transformation system using cotyledon, hypocotyl, and leaf explants. The process is reliable, reproducible, and highly customizable depending on research goals.
| Step | Description | Deliverable |
| Explant preparation | Sterile cotyledon, hypocotyl, or leaf tissue | High-quality explants |
| Agrobacterium preparation | Transformation of vectors into A. tumefaciens | Stable Agrobacterium suspension |
| Infection & co-cultivation | Explants incubated with Agrobacterium | T-DNA integration into the genome |
| Selection | Antibiotic/herbicide resistance screening (Kana, Hyg, Basta) | Positive calli or shoots |
| Regeneration | Tissue differentiation and shoot/root induction | Transgenic plants |
| Molecular characterization | PCR, sequencing, phenotyping (optional) | Validated T0 plants |
| Seed harvesting | Self-pollination and T1 seed collection | T1 generation lines |
Creative Biogene provides flexible transformation solutions, ranging from standard stable transformation to advanced genome editing.
| Service Type | Deliverables | Timeline |
| Standard transformation | ≥5–10 independent positive lines | 3–4 months |
| CRISPR/Cas-mediated editing | ≥3 confirmed edited T0 lines, sequencing validation | 3–5 months |
| Base editing (ABE/CBE/PE) | Edited lines with mutation details | 3–6 months |
| Customized cloning | Expression, RNAi, amiRNA, CRISPR/Cas vectors | 7–15 days |
We also offer customized project design, including:
Creative Biogene has experience in transforming a wide spectrum of tomato ecotypes, elite cultivars, and wild accessions, including:
Note: Common varieties are maintained in-house and can be used year-round for transformation projects.
Upon completion, clients receive:
| Parameter | Details |
| Explant types | Cotyledon, hypocotyl, leaf |
| Transformation method | Agrobacterium-mediated |
| Selective markers | Kana, Hyg, Basta |
| Turnaround time | 3–6 months (depending on service type) |
| Deliverables | ≥5–10 positive T0/T1 lines, with full molecular validation |
At Creative Biogene, we combine scientific expertise, advanced plant tissue culture facilities, and client-oriented project management to ensure reliable, efficient, and reproducible results. Whether you are conducting basic plant functional studies or working toward crop trait improvement, our tomato transformation service provides the right solution. Contact us today for consultation and customized project design. Let our experts accelerate your tomato genetics research and crop innovation.
Copyright © Creative Biogene. All rights reserved.