Pages
Products
Services
CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER

Strawberry Transformation Service

Strawberry is a globally popular, high-value horticultural crop whose complex genomic structure and heterogeneity significantly constrain genetic research and varietal improvement. Leveraging extensive experience and advanced technologies in plant transformation, Creative Biogene provides efficient, professional strawberry genetic transformation services with diverse technical solutions covering both the model species F. vesca and the cultivated F. × ananassa, enabling research clients to efficiently achieve functional validation and targeted trait improvement.

Strawberry (Fragaria × ananassa), one of the world's most beloved berry fruits, occupies a prominent position in both fresh produce and processed food markets due to its vibrant color, rich aroma, distinctive flavor, and nutritional value. Rich in vitamin C, anthocyanins, polyphenols, and various minerals, strawberries offer significant antioxidant and health benefits, maintaining a broad consumer base globally. Commercial strawberry cultivation primarily focuses on hybrid octoploid populations (modern cultivated Fragaria × ananassa), which originated from natural hybridization between the North American wild species F. chiloensis and F. virginiana. Its complex genomic structure (octoploid, 2n = 8x = 56) not only exhibits high redundancy in gene expression and genetic control but also presents formidable challenges for genetic improvement.

Traditional breeding approaches are limited in efficiency for enhancing strawberry quality, resistance, and adaptability due to long hybridization cycles, severe phenotypic segregation in progeny, complex genetic backgrounds, and difficulties in dominant/recessive gene identification. With the rapid development of genetic engineering, transformation technology demonstrates irreplaceable advantages in strawberry variety improvement. Establishing stable genetic transformation systems enables precise introduction of target genes and control of specific trait expression, significantly accelerating strawberry functional gene analysis and molecular breeding processes.

Strawberry Transformation Services Profile

Creative Biogene is dedicated to establishing efficient, stable, and reproducible strawberry genetic transformation platforms. Leveraging our profound understanding of octoploid strawberry biological characteristics and genotypic differences, combined with optimized tissue culture systems, improved vector design strategies, and diverse molecular verification methods, we comprehensively enhance strawberry genetic transformation technology, enabling researchers and breeding specialists to achieve breakthroughs in functional gene research, precise trait improvement, and new variety development.

Genetic Transformation System Development

As strawberry tissue culture processes are highly genotype-dependent, Creative Biogene has developed proprietary genetic transformation systems with optimized workflows for callus induction, bud differentiation, and regeneration. Our commonly employed transformation methods include:

  • Agrobacterium-mediated stable transformation
  • Transient fruit transformation system
  • Hairy root transformation system
  • Protoplast transient expression system

Gene Overexpression and Silencing Services

To meet diverse client requirements in functional gene research, we provide gene overexpression and RNA interference (RNAi)/virus-induced gene silencing (VIGS) technical services for strawberry:

  • Overexpression vector design and construction (multiple promoter options, including 35S, UBQ, fruit-specific promoters, etc.)
  • RNA interference fragment design and vector construction
  • TRV-VIGS system application optimization for strawberry leaf and fruit tissues
  • Molecular detection of expression materials (qRT-PCR, Northern blot, Western blot)

Gene Editing Services

Despite challenges in polyploid crop gene editing, we have established CRISPR/Cas system platforms suitable for strawberry, providing comprehensive services in:

  • Genomic analysis and gRNA site screening (based on F. × ananassa and F. vesca)
  • CRISPR/Cas9 or Cas12a system vector construction
  • Transformation and tissue regeneration process optimization
  • Multi-locus knockout and editing verification
  • Transgenic/non-transgenic progeny segregation strategies

Service Application Directions

Leveraging our genetic transformation and gene editing platforms, Creative Biogene can assist clients in achieving the following major trait improvement objectives:

  1. Fruit quality enhancement: Including sugar-acid ratio and aroma compound synthesis-related gene regulation
  2. Non-climacteric ripening regulation: Validation of key regulatory genes in ABA signaling pathways and transcription factors
  3. Storage durability and firmness enhancement: Silencing or mutation of cell wall metabolism-related enzyme coding genes
  4. Disease resistance improvement: Introduction or activation of genes related to resistance against gray mold, anthracnose, etc.
  5. Environmental stress adaptation: Functional analysis of drought, high temperature, salt stress response genes

Service Process Overview

  1. Project consultation and requirements confirmation
  2. Explain source selection and pretreatment optimization
  3. Vector construction and verification
  4. Transformation operation and tissue culture
  5. Positive plant screening and validation
  6. Functional verification and phenotypic analysis
  7. Project summary and results delivery

Contact Us

If you are conducting strawberry-related functional gene research, trait improvement, or molecular breeding projects, Creative Biogene is your trusted technical partner. We possess professional transformation platforms, mature experimental systems, and efficient client support mechanisms to advance your research efficiently. Please contact us anytime for more customized solutions and quotations.

References:

  1. Liu Z, Liang T, Kang C. Molecular bases of strawberry fruit quality traits: Advances, challenges, and opportunities. Plant Physiol. 2023;193(2):900-914.
  2. Vondracek K, Altpeter F, Liu T, et al. Advances in genomics and genome editing for improving strawberry (Fragaria ×ananassa). Front Genet. 2024;15:1382445.
* For research use only. Not intended for any clinical use.
Quick Inquiry