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Ipomoea batatas Transformation Service
Equipped with state-of-the-art instrumentation, Creative Biogene focuses on the exploration and utilization of genetic engineering technologies to generate transgenic plants to accelerate plant biology research and crop improvement. We offer a complete Ipomoea batatas transformation service ranging from gene synthesis/cloning, construct design, Agrobacterium-mediated transformation, expression evaluation, functional analysis to phenotypic analysis. Based on excellent platform and leading-edge technology, our experienced scientist team serves our customers with high-quality services.
Sweet potato, Ipomoea batatas (L.) Lam., is an important food and industrial material crop in the world. It is also an alternative source of bio-energy as a raw material for fuel production. The improvement of this crop by conventional hybridization is limited because of its high male sterility, incompatibility and the hexaploid nature. Gene engineering, especially genetic transformation offers great potential for the improvement of sweet potato. Creative Biogene has established an efficient system of embryogenic suspension cultures for sweet potato genotypes especially for commercial cultivars. Using embryogenic suspension cultures of sweet potato, we have also established an efficient Agrobacterium tumefaciens-mediated transformation system and obtained transgenic plants.
Figure1. Agrobacterium-mediated sweet potato transformation using embryogenic suspension cultures
Workflow of sweet potato transformation
- Shoot apical meristem (SAM) isolation
- Induction culture of SAM to generate embryogenic callus
- Embryogenic suspension cultures generation
- Agrobacterium infection and co-cultivation
- Selection and differentiation
- Transgenic plantlets regeneration
Detail information of sweet potato transformation
- Variety: Taizhong 6, Ayamurasaki, Xushu 22, Xushu 18, Jishu 26
- Selection system: Hygromycin
- Customized transformation-related service: vector design and construction, genome editing.
- Expression evaluation: RT-qPCR, Western blot
With superior expertise in plant biotechnology, Creative Biogene offers one-stop sweet potato transformation service for global clients. Our complete service including project consulting, construct design, genetic transformation, molecular characterization and functional analysis. Besides, Creative Biogene is confident to handle genome editing project, with years of experience in using CRISPR/Cas9.
Reference:
- Sohrab, S. (2016). Development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene. Saudi Journal of Biological sciences, 23, 358.
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About Us
Creative Biogene is a US-based manufacturer and provider of genomics and proteomics products and services for academic and governmental research institutes, pharmaceutical and biotechnology industry.
