Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Apple is a core component of the global fruit industry and one of the most economically important temperate fruit trees. Creative Biogene has developed a comprehensive genetic transformation service system for apple, covering stable genetic transformation, transient expression, gene function research, and gene editing across multiple directions. We develop specialized technical approaches for different cultivars such as "Fuji" and "Gala," enabling researchers to efficiently explore the apple's genetic foundations and trait improvement pathways.
Apple (Malus × domestica Borkh.) has extensive cultivation and commercial value worldwide, with breeding and trait improvement research being crucial for increasing yield, enhancing quality, and strengthening stress resistance. As an outcrossing perennial woody plant with a complex genome structure and long life cycle, traditional breeding methods often require multiple generations of backcrossing, screening, and phenotypic identification, resulting in lengthy breeding cycles, low efficiency, and unstable genetic backgrounds that cannot meet the rapid development needs of modern agriculture. Additionally, genetic transformation faces numerous technical challenges due to issues such as browning during tissue culture, low callus induction efficiency, and unstable regeneration rates. In recent years, with advances in plant tissue culture techniques, improved Agrobacterium-mediated transformation efficiency, and continuous optimization of gene editing tools, apple genetic transformation has gradually evolved from "technical exploration" to "application breakthrough."
Leveraging our advanced plant transformation platform and extensive experience with woody fruit tree transformation, Creative Biogene provides comprehensive apple genetic transformation solutions covering Agrobacterium tumefaciens-mediated stable transformation systems, virus-induced gene silencing (VIGS), virus-mediated overexpression (VOX), artificial microRNA (amiRNA)-mediated silencing systems, and CRISPR/Cas9 gene editing platforms.
1. Stable Transformation System
Currently, the leaf disc method mediated by Agrobacterium tumefaciens remains the most efficient and widely applied stable genetic transformation approach for apple. Creative Biogene has continuously optimized transformation conditions and regeneration systems to develop a highly efficient transformation process applicable to multiple varieties.
We offer the following support:
2. Gene Silencing and Overexpression Systems
To achieve functional loss or enhanced expression of specific genes, Creative Biogene provides various reversible regulatory strategies:
3. Gene Editing Platform
To address the need for knockout or precise editing of multiple functional genes in apple, we have established gene editing platforms:
4. Transformation System Development and Cultivar Adaptability Optimization
One of the challenges in apple transformation systems is their strong dependence on variety. Creative Biogene has developed customized explant processing strategies and culture media systems for varieties with different genetic backgrounds and regeneration capabilities. We also combine phenotypic screening with transcriptome analysis at key regeneration stages to provide a basis for developing proprietary transformation systems for new varieties.
For more information or customized service solutions, please contact Creative Biogene's plant genetic transformation technology team. We will provide you with one-stop, professional Apple transformation solutions.
References:
Copyright © Creative Biogene. All rights reserved.