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Creative Biogene offers professional genetic transformation services for mustard (Brassica juncea), designed to enhance disease resistance, stress tolerance, yield, and nutritional quality through advanced Agrobacterium-mediated methods and gene editing technologies. Addressing current disease threats and genetic improvement challenges in mustard, we provide standard transformation services and customized optimization systems for special varieties, supporting gene overexpression, gene silencing, and precise genome editing.
Mustard (Brassica juncea) is an important oilseed crop in the Brassicaceae family, widely cultivated for edible oil production. It is a natural amphidiploid formed by hybridization between B. rapa and B. nigra, originating approximately 39,000-55,000 years ago. Global mustard cultivation area and production are substantial, with India being one of the major producers. However, due to genomic uniformity among varieties, mustard is susceptible to various diseases (such as Alternaria blight, Sclerotinia stem rot, etc.), which pose serious threats to global mustard production. Current cultivars lack vertical and horizontal resistance to these pathogens, resulting in poor control effectiveness. Although chemical pesticides are used for disease management, this approach is not sustainable due to climate change impacts and economic constraints. Disease control primarily relies on genetic resistance, with disease-resistant genes from wild populations providing important resources for improving mustard disease resistance. With advances in agricultural biotechnology, genetic improvement research in mustard has gradually deepened, and genetic transformation technology has received increasing attention as an important means to improve resistance, yield, and nutritional value.
Creative Biogene's services include a complete technical process from gene introduction to plant regeneration, root culture, and molecular detection of transgenic plants, aiming to provide customers with efficient and reliable genetic improvement solutions.
Mustard Genetic Transformation System Development
Creative Biogene provides customized transformation system development services for different mustard varieties. Through optimization of Agrobacterium-mediated gene transformation technology, we can efficiently introduce target genes for multiple traits, ensuring optimal transformation conditions (such as strain, medium, hormone ratio, and infection time) to meet customer needs.
Gene Overexpression and Silencing
We provide mature gene overexpression and silencing technologies to regulate specific gene expression levels and improve mustard traits. Examples include overexpressing disease-resistant genes to enhance resistance or using RNA interference technology to silence undesirable traits (such as bitterness, drug resistance).
Gene Editing
Using CRISPR/Cas9 technology to precisely edit mustard genes, improving traits such as drought tolerance, insect resistance, and disease resistance without introducing foreign genes, and achieving precise genome modification.
Genetic Improvement and Variety Optimization
Combining genetic transformation and molecular marker technologies, Creative Biogene helps customers develop new varieties, enhancing mustard's resistance to pests and diseases, yield, quality, and other characteristics, promoting sustainable industry development.
Transgenic Plant Verification and Screening
We provide comprehensive verification services for transgenic plants, including genotype and phenotype verification, stability testing, PCR, Southern hybridization, and other technologies to ensure high-quality standards for transformed plants.
Creative Biogene's Mustard Greens Transformation Service provides researchers and enterprises with an efficient and reliable genetic improvement tool. Through precise operations and strict quality control, we are committed to providing customers with the highest quality transformation results, promoting innovation in agricultural biotechnology. If you have any needs or questions, please feel free to contact us at any time, and we will provide you with professional support.
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