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Brassica Oleracea Transformation Service

Creative Biogene offers a complete Brassica transformation service from experimental design and construct assembly through to transformation and screening of the plants developed. Our platform can help to advance research in many areas of plant science by providing functional characterization of genes of interest and by providing knock-out mutants using CRISPR/Cas9 based technologies.

Brassica oleracea, a species commonly known for its polymorphism, comprises a number of varieties, including cabbage, broccoli, cauliflower, kale, Brussels sprouts, collard greens, Savoy cabbage, kohlrabi, and gai lan. Genetic transformation technology is vital to gene discovery, functional analyses and variety improvement. It has allowed the development of novel Brassica varieties, such as those producing nutriceutical, biodegradable plastic or biopharmaceuticals, by introducing novel genes from unrelated sources. Thus, an efficient and rapid transformation system will facilitate the genetic improvement and breeding of Brassica oleracea.

Workflow

  • Seed sterilization and germination
  • Preparation of Agrobacterium suspension
  • Explant isolation, inoculation and co‐cultivation
  • Regeneration of transgenic shoots
  • Selection culture
  • Root initiation
  • Transfer of plants to greenhouse

Procedures of Agrobacterium-mediated Brassica oleracea transformation. Figure 1. Procedures of Agrobacterium-mediated Brassica oleracea transformation.

Advantages

  • Fully matured plant genetic engineering platform.
  • Professional teams in molecular construction, tissue culture, transformation and phenotypic analysis.
  • Years of experience in crop genome editing using CRISPR/Cas9.
  • Fast turnaround time and affordable price.

Experienced scientists and experts of Creative Biogene will work closely with you to make sure your project is given the best intellectual and technical support. For more information of service details, questions or collaborations, please feel free to contact us.

Reference:

  1. Liu, C. W. (2007). Stable chloroplast transformation in cabbage (brassica oleracea l. var. capitata l.) by particle bombardment. Plant Cell Reports, 26(10), 1733-1744.
For research use only. Not intended for any clinical use.

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