Solanum melongena Transformation Service
Eggplant, as one of the world's important economic crops, has a wide cultivation base and genetic diversity globally. Combining years of plant molecular breeding experience, Creative Biogene has established an efficient and stable eggplant genetic transformation platform, providing customized technical services for basic research and commercial breeding projects, including variety-specific transformation system development, functional gene analysis, gene editing, and trait improvement.
Eggplant (Solanum melongena L.), belonging to the Solanaceae family, genus Solanum, is one of the world's six most important vegetable crops, with global annual production exceeding 50 million tons, primarily concentrated in China, India, Turkey, Egypt, and Southeast Asian countries. As a self-pollinating crop, eggplant has a relatively narrow genetic background, making the in-depth exploration and utilization of germplasm resources urgently dependent on modern molecular biology tools for precise and efficient genetic improvement.
Due to its relatively large floral structure and ease of artificial pollination, eggplant is also an important material for heterosis research and hybrid breeding. In recent years, the continuous advancement of genome sequencing and functional genomics research has provided rich information resources for eggplant molecular breeding and gene function research. Particularly in disease resistance, stress tolerance, yield, and quality improvement, the introduction of beneficial genes through genetic transformation technology has become a trend.
Service Content
Creative Biogene provides comprehensive and systematic eggplant genetic transformation services, covering multiple levels from basic research to commercial breeding applications, including:
- Eggplant Agrobacterium-mediated Genetic Transformation System Establishment and Optimization
- Development of specialized, efficient transformation systems for different genetic backgrounds and cultivated varieties (especially landraces and breeding varieties)
- Precise regulation of culture conditions, hormone ratios, and regeneration systems
- Gene Overexpression and RNAi/Artificial miRNA Gene Silencing
- Functional gene validation and trait regulation research
- Use of promoter-driven tissue-specific or inducible expression vectors
- Gene Editing Services
- Development of site-specific mutation materials
- Multi-site gene knockout/knock-in strategy design
- Editing efficiency detection and off-target analysis
- Beneficial Trait Introduction and Exogenous Gene Expression Analysis
- Introduction and expression regulation of target trait genes for disease resistance, stress tolerance, fruit quality, plant morphology, etc.
- Bt protein expression for insect resistance, introduction of abiotic stress-related genes (e.g., salt tolerance, drought resistance)
- Transformed Plant Screening and Molecular Detection
- Resistance screening markers (glyphosate resistance, kanamycin resistance, etc.)
- Verification by PCR, qRT-PCR, Southern blot, Western blot, and other methods
- Activation Tagging Mutant Library Construction
- Construction of gene activation mutant populations through transgenic methods to facilitate functional gene mining
Technical Advantages
- Rich Platform Experience: Accumulated extensive transformation experience with eggplants of different genetic backgrounds and varieties
- Efficient Transformation System: High regeneration rate, low contamination rate, high positive rate
- Wide Range of Transformation Varieties: Support for multiple local and modern varieties including oblong, oval, purple-skinned, green-skinned, etc.
- Customized Services: Can conduct transgenic, gene editing, or mutant library construction according to client requirements
- Strict Quality Control: Detailed experimental records and quality control standards at each step
Application Directions
- Gene function research
- Heterosis mechanism exploration and accelerated hybrid breeding
- Disease and pest resistant variety development (e.g., Fusarium wilt resistance, eggplant fruit and shoot borer resistance)
- Fruit quality improvement (e.g., antioxidant content, thin skin texture)
- Stress tolerance trait improvement (salt tolerance, drought resistance, heat tolerance)
- Development of thornless eggplant new lines
- Genetic engineering product development and new material validation
Service Process
Requirement Communication → Experimental Design → Material Preparation → Transformation Implementation → Molecular Verification → Delivery Report → Follow-up Support
Contact Us
Creative Biogene is committed to providing high-quality, reproducible plant transformation solutions for researchers and seed companies. Contact us for customized eggplant genetic transformation solutions and quotations.
Case Study
References:
- Kumar A, Sharma V, Jain BT, et al. Heterosis Breeding in Eggplant (Solanum melongena L.): Gains and Provocations. Plants (Basel). 2020;9(3):403. Published 2020 Mar 24.
- Berghi ON, Vrinceanu D, Cergan R, et al. Solanum melongena allergy (A comprehensive review). Exp Ther Med. 2021;22(4):1061.
* For research use only. Not intended for any clinical use.
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