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Creative Biogene has developed transient and stable transformation systems within the model plant tobacco, specialized for your needs, including gene function analysis, gene expression regulation, protein subcellular localization, promoter activity evaluation, protein-protein interaction analysis and the development of plant bioreactors. With a lot of talent scientists and technicians, we have assisted in hundreds of plant transformation projects for global clients. In addition, we will also save your time and cost by taking advantage of our expertise and innovative technology platforms.
Tobacco is a rich source of the alkaloid nicotine and has a long history of use as herbal medicine and social stimulant. It has been playing a crucial role in molecular farming and offering several practical advantages over other crops. It produces significant leaf biomass, has high soluble protein content and is a non-food crop, minimizing the risk of food-chain contamination. This, combined with its flexibility and highly-efficient genetic transformation/regeneration, has made tobacco become a model system of plant biology research and particularly well suited for plant-based production of biopharmaceutical products.
Agrobacterium-mediated leaf disk transformation is a genetically stable method which is widely used in transgenic plants creation with high efficiency and relatively short turnaround time. At Creative Biogene, we improve the transformation protocol to make it more suitable for different genotypes. In addition, we introduce a transient transformation system which can be realized by injecting engineered Agrobacterium into the abaxial surface of the tobacco leaf. It can be used in protein subcellular localization and protein-protein interaction.
Chloroplast genome transformation offers multiple advantages over nuclear genome which among others, such as eliminating gene silencing and position effect, higher level of transgene expression and significant reduction in environmental dispersion of the transgene due to maternal inheritance. Creative Biogene establishes a chloroplast transformation system which is usually divided into four steps: 1) The transformation vector carrying the foreign gene is introduced into the chloroplast by biolistics or other transformation system. 2) The exogenous gene expression frame is integrated into the chloroplast genome by homologous recombination. 3) Screening for successfully transformed chloroplast cells. 4) Subculture to obtain stable chloroplast transformed plants.
Creative Biogene is a professional expert in plant transformation with a variety of mature platforms and technologies for the improvement of transformation efficiency. Especially, we offer transformation service of tobacco chloroplast for production of vaccine antigens, antibodies for immunotherapy, nutritional supplements and enzymes. We are well equipped to handle all matters you are suffering and provide you the best services.
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