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Arabidopsis thaliana has long been recognized as the most important model plant in modern plant biology and molecular genetics. Its short life cycle, small genome, and ease of cultivation make it an indispensable system for functional gene studies, dissecting regulatory pathways, and developing genome editing technologies. Creative Biogene, backed by years of technical expertise, offers a professional and highly efficient Arabidopsis transformation platform that supports global researchers in accelerating their plant science projects.
Figure 1. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method.
Unlike many other crop species that require lengthy and complex transformation protocols, Arabidopsis provides a rapid, reliable, and cost-effective system. Its self-pollination ability facilitates stable line propagation, while the widely used Agrobacterium-mediated floral dip method enables straightforward gene delivery without tissue culture steps.
Researchers rely on Arabidopsis transformation for:
Creative Biogene adopts the optimized Agrobacterium tumefaciens GV3101–mediated floral dip method, which ensures high reproducibility and transformation efficiency. The core steps include vector construction, Agrobacterium preparation, floral dip inoculation, seed selection, and stable line generation.
To make the process transparent, our workflow is summarized below:
| Step | Description | Expected Outcome |
| Project consultation | Review construct, design experiment | Optimized plan tailored to your needs |
| Vector preparation | Construct expression vector or use the client-provided | Verified plasmid ready for transformation |
| Agrobacterium inoculation | Transform the vector into A. tumefaciens and prepare culture | Stable Agrobacterium strains |
| Floral dip transformation | Dip Arabidopsis flowers in bacterial suspension | T-DNA delivery into ovules |
| Seed selection | Grow T1 seeds on selective medium | Positive seedlings identified |
| Line confirmation | Genotyping, sequencing, phenotyping (optional) | Stable transgenic or edited Arabidopsis lines |
| Delivery | Shipment of seeds and a full report | Independent positive T1/T2 lines |
This streamlined workflow minimizes variability and ensures reliable delivery of transgenic plants.
| Parameter | Details |
| Ecotypes | Col, Ws, Ler |
| Timeline | 4–5 months (T1 seeds), 6–7 months (T2 homozygous) |
| Transgenic events | ≥10 independent lines guaranteed |
| Editing efficiency | Up to 80% (CRISPR) |
| Capacity | ~900 constructs annually |
| Facilities | Air shower, clean bench, Bio-Rad PDS-1000/He, fluorescent microscopy, greenhouse |
Unlike general contract labs, Creative Biogene provides end-to-end solutions with strong guarantees of quality and efficiency:
Upon completion, you will receive:
Deliverables can be tailored depending on whether you require standard transformation or full CRISPR editing packages.
Arabidopsis transformation services provided by Creative Biogene have been applied in:
By bridging model plant research with agricultural biotechnology, our services support both academic and industrial clients.
Choosing Creative Biogene means working with a team that combines scientific expertise, advanced facilities, and customer-oriented project management. We not only deliver seeds but also provide insightful analysis and flexible customization, ensuring that your project runs smoothly from conception to completion.
Our clients value:
Whether you need standard transformation, genome editing, or customized solutions, Creative Biogene is your trusted partner for Arabidopsis thaliana transformation services. Contact us today to discuss your project. Our experts will provide a complimentary consultation and design the most effective path to achieving your research objectives.
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