Petunia Transformation Service
Petunia is not only a widely cultivated ornamental plant worldwide but also an important model for plant genetic engineering research. Based on a deep understanding of the petunia genetic background and tissue regeneration systems, Creative Biogene has built a systematic, high-efficiency genetic transformation platform to support researchers in achieving breakthrough results in new variety creation, functional gene validation, and molecular mechanism studies.
Petunia belongs to the Petunioideae subfamily of Solanaceae and is native to South America as an herbaceous plant. Due to its short life cycle, easy seed propagation, strong regeneration ability, and simple tissue culture operation, it became one of the earliest flowering plants used in genetic engineering breeding research. Its well-defined genetic background and mature tissue culture system provide an ideal platform for functional gene research and molecular breeding. Currently, petunia has been widely applied in transgenic flower development, flower color regulation mechanism research, flowering regulation, plant secondary metabolism, and stress response studies. Research depth now covers the entire process from protoplast transformation and haploid induction to molecular identification, making it an important material for plant somatic breeding and transgenic technology validation.
Service Content
Creative Biogene is dedicated to providing multi-level, comprehensive support for gene function research and trait improvement in petunia genetic transformation services. Our service scope includes not only conventional transgenic plant construction but also the following specialized directions:
- Genetic Transformation System Optimization and Construction: For different cultivation types or specific mutant backgrounds of petunia varieties (especially commercial floriculture systems), we can customize and develop transformation systems, including selecting appropriate Agrobacterium strains, regeneration system optimization, and resistance screening system improvements to enhance transformation efficiency and shorten cycles.
- Exogenous Gene Overexpression and Silencing (RNAi/antisense) Strategies: We can achieve precise regulation of target genes. For example, overexpression of the EPSP synthase gene confers high glyphosate tolerance to transgenic plants, or by constructing antisense structures, effectively delaying senescence, postponing flower and leaf degradation, and extending the commercial period.
- Gene Editing Service: Combined with the latest editing tools, we can perform knockout, knock-in, or point mutation operations on key metabolic pathway-related genes (such as anthocyanin synthesis, ethylene synthesis, etc.), facilitating functional validation and new variety creation, particularly suitable for rapidly obtaining trait mutation materials.
- Flower Color and Ornamental Trait Genetic Improvement: In multiple successful cases, we have achieved the creation of new flower colors ranging from brick red and orange-red to white and variegated patterns, significantly expanding petunia's flower color phenotypes and bringing considerable added value to the horticultural industry.
- Multi-gene Complex Editing and Metabolic Pathway Reconstruction: Combined with metabolic engineering approaches, we can perform complex regulation of multiple control points in flavonoid synthesis, fragrance synthesis, and other pathways.
- Trait Validation and Phenotype Evaluation: Our complementary greenhouse platform can systematically evaluate improved petunia plants, including drug resistance verification, flowering cycle, color saturation, leaf longevity, transcription expression levels, etc., ensuring that clients obtain stable and scalable transgenic or edited materials.
Technical Advantages
- Mature Tissue Culture System: Years of accumulated experience in petunia regeneration system optimization ensure high-frequency bud differentiation and seedling efficiency.
- Flexible Transformation Solutions: Selection systems (Km, Hyg, Bar, etc.) can be chosen according to the client gene characteristics.
- Efficient Screening and Verification Process: Standardized molecular detection platform supports rapid identification of positive lines, saving verification cycles.
- Rich Explant Source Options: Support for multiple explant types, including seeds, leaves, stem segments, and petals, adapting to different experimental needs.
- Customized Service Support: Can be extended to transcriptome analysis, metabolite detection, stable expression analysis, and other value-added modules.
Application Directions
- New Variety Development: Achieve petunia flower color, fragrance, stress resistance, flowering period, and other trait improvements through exogenous gene introduction.
- Gene Function Research: Validate the biological functions of genes regulating flowering, flower color, hormone synthesis, and stress response.
- Molecular Breeding Screening Platform Construction: Evaluate candidate gene functions based on high-throughput screening systems.
- Epigenetic Research: Combine transgenic materials for promoter activity detection and regulatory element functional verification.
- Teaching and Demonstration: Provide standard processes and platform support for genetic transformation technology training and teaching experiments.
Service Process
- Project Communication: Clarify research objectives, determine target genes, and expression strategies
- Material Preparation: Clients provide target genes, or we complete vector construction
- Genetic Transformation: Conduct transformation experiments and monitor bud differentiation and positive screening
- Plant Cultivation: Obtain resistant plants and complete rooting, transplanting, and acclimation
- Molecular Identification: Perform molecular-level confirmation of transgenic plants
- Data Reporting: Provide complete experimental reports, process diagrams, and relevant data
Case Study
Contact Us
If you have specific gene transformation needs or require further customization of certain processes, Creative Biogene looks forward to providing you with one-stop plant genetic engineering solutions. For inquiries, please contact our plant transformation service team.
References:
- Strazzer P, Verbree B, Bliek M, et al. The Amsterdam petunia germplasm collection: A tool in plant science. Front Plant Sci. 2023 Mar 21;14:1129724.
- Parmar N, Singh KH, Sharma D, et al. Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review. 3 Biotech. 2017;7(4):239.
* For research use only. Not intended for any clinical use.
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