Coix lacryma-jobi Transformation Service
Creative Biogene has established a dedicated genetic transformation platform for Coix, which facilitates the precise incorporation of beneficial traits and enables efficient genome manipulation for research and breeding purposes. This service integrates proven methodologies such as Agrobacterium-mediated transformation, genome editing, and optimized plant regeneration systems to empower functional genomics studies, metabolic engineering, and precision breeding in Coix.

Coix lacryma-jobi L., commonly known as Job's tears, is a C4 plant in the Poaceae family, widely cultivated across Southeast Asia, South Asia, and Southwestern China. Recognized for both its nutritional and medicinal properties, Coix seeds are rich in bioactive compounds with anti-cancer, anti-inflammatory, antioxidant, and other health-promoting effects. Despite its potential as a functional food and herbal medicine, industrial development of Coix remains hindered by issues such as limited cultivar diversity, susceptibility to disease, low yield, and underutilization of non-seed tissues.
Our Service Portfolio
Creative Biogene provides end-to-end genetic transformation services for Coix, covering the full pipeline from vector design to stable transgenic line development and molecular validation.
| Project Type | Technical Details |
| Transformation System Development | Establishe high-efficiency transformation systems for various Coix cultivars and genotypes. Select optimal callus types and Agrobacterium strains (e.g., EHA105, AGL1), and optimize parameters such as bacterial density, co-cultivation conditions, and antibiotic selection. |
| Gene Overexpression / Gene Silencing | Introduce target genes or RNAi constructs via Agrobacterium tumefaciens-mediated transformation to regulate gene expression in Coix. |
| Genome Editing | Design targeted editing constructs for site-specific knockouts/knock-ins in the Coix genome, supporting multiplex editing, directed mutagenesis, and trait stacking. |
| Stable Transgenic Plant Generation | Refine regeneration protocols using hormone combinations (e.g., KT, 6-BA, GA₃) to obtain healthy, stable transgenic Coix lines with full molecular and phenotypic validation. |
| Genotype-Specific Transformation Optimization | Develop customized transformation protocols for elite cultivars, including tailored callus induction strategies from explants such as adventitious roots, immature embryos, or stem segments. |
| Trait Improvement Project Support | Offer end-to-end support for breeding projects, from gene screening and vector construction to transformation and phenotypic evaluation, facilitating directed trait improvement and molecular breeding implementation. |
Key Advantages of Coix lacryma-jobi Transformation Service
- Extensive Experience in Monocot Transformation: Proven expertise in transforming cereal crops such as rice, sorghum, and maize enables rapid adaptation to diverse Coix genotypes.
- High-Quality Callus Selection System: Establishes standardized criteria to evaluate callus quality (e.g., color, texture, compactness), ensuring high responsiveness and transformation efficiency.
- Integrated Infection Enhancement Strategies: Combines physical and chemical methods (e.g., vacuum infiltration, ultrasound treatment, phenolic compound induction) to improve Agrobacterium uptake and transformation success.
- Customized Project Design: Tailors transformation strategies to specific research goals, including gene functional analysis, trait improvement, or biosynthetic pathway reconstruction, ensuring scientific rigor and safety.
- Comprehensive Molecular Validation: Provides full molecular assessment of transgenic lines, including PCR, qPCR, GUS staining, Southern blot, sequencing, and expression analysis.
Applications
- New Variety Development: Introduces genes related to disease resistance, stress tolerance, yield improvement, and quality enhancement to breed high-performance Coix cultivars.
- Metabolic Engineering Research: Enables the study of regulatory mechanisms behind bioactive compounds (e.g., coixenolide, phenolic acids), supporting the development of functional products.
- Gene Function Discovery: Supports functional genomics and candidate gene analysis through transgenic and gene-edited Coix platforms.
- Precision Breeding System Establishment: Integrates marker-assisted selection and genome editing to build efficient and precise Coix genetic improvement pipelines.
Case Study

Contact Us
If you are interested in developing a genetic transformation system for Coix, constructing transgenic lines, or launching a customized genetic improvement project, please contact the Plant Molecular Platform at Creative Biogene. We are committed to providing reliable, efficient, and scientifically driven one-stop solutions to accelerate your research goals.
References:
- Weng WF, Peng Y, Pan X, et al. Adlay, an ancient functional plant with nutritional quality, improves human health. Front Nutr. 2022;9:1019375.
- Meng FD, Yuan L, Lu DD, et al. Anti-tumor effect of coix seed based on the theory of medicinal and food homology. World J Clin Oncol. 2023;14(12):593-605.
* For research use only. Not intended for any clinical use.